Venus-A2AR vector

Venus-A2AR construct was generated with the In-fusion HD Cloning Kit (Clontech Takara, USA). Venus and A2AR fragments were produced by using, respectively, the pair of primers 5′-GTTTAAACTTAAGCTTATGGTGAGCAAGGGCGAG-3′ and 5′-GCTGCCCATGGTGGCCTTGTACAGCTCGTCCATG-3′, and the pair of primers 5′-GCCACCATGGGCAGCAGC-3′ and 5′-AAACGGGCCCTCTAGATCAGCTGGGGGCGAACTC-3′. PCR fragments were cloned into the vector pcDNA3.1( + ) linearized with HindIII and XbaI, and the resulting construct was […]

Contributors

Inventor Institute
LuĂ­sa V Lopes Instituto de Medicina Molecular JoĂŁo Lobo Antunes
Cat. #: 160728
Tool sub type: pcDNA 3.1
Research Fields: Cell biology;Neurobiology
Target: Adenosine A2A receptor
Bacterial resistance: Ampicillin
Selectable markers: Neomycin
Alternate name: A2AR
Product description: Venus-A2AR construct was generated with the In-fusion HD Cloning Kit (Clontech Takara, USA). Venus and A2AR fragments were produced by using, respectively, the pair of primers 5'-GTTTAAACTTAAGCTTATGGTGAGCAAGGGCGAG-3' and 5'-GCTGCCCATGGTGGCCTTGTACAGCTCGTCCATG-3', and the pair of primers 5'-GCCACCATGGGCAGCAGC-3' and 5'-AAACGGGCCCTCTAGATCAGCTGGGGGCGAACTC-3'. PCR fragments were cloned into the vector pcDNA3.1( + ) linearized with HindIII and XbaI, and the resulting construct was verified by DNA sequencing (GATC Biotech, Germany).
Additional notes: A construct encoding a Venus-A2AR fusion protein to explore the synaptic function of A2AR
Application notes: Venus-A2AR construct was generated with the In-fusion HD Cloning Kit (Clontech Takara, USA). Venus and A2AR fragments were produced by using, respectively, the pair of primers 5'-GTTTAAACTTAAGCTTATGGTGAGCAAGGGCGAG-3' and 5'-GCTGCCCATGGTGGCCTTGTACAGCTCGTCCATG-3', and the pair of primers 5'-GCCACCATGGGCAGCAGC-3' and 5'-AAACGGGCCCTCTAGATCAGCTGGGGGCGAACTC-3'. PCR fragments were cloned into the vector pcDNA3.1( + ) linearized with HindIII and XbaI, and the resulting construct was verified by DNA sequencing (GATC Biotech, Germany).
References:

Temido-Ferreira et al. 2020. Mol Psychiatry. 25(8):1876-1900. PMID: 29950682.

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