Adapted from Temido-Ferreira et al.; 2020. Mol Psychiatry; 25(8)

Cat. #160728

Venus-A2AR vector

Cat. #: 160728

Sub-type: pcDNA 3.1

Availability: Please enquire for quantities and pricing

Target: Adenosine A2A receptor

Bacterial Resistance: Ampicillin

Selectable Markers: Neomycin

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

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Contributor

Inventor: LuĂÂĂÂsa V Lopes

Institute: Instituto de Medicina Molecular JoĂÂÄšÂo Lobo Antunes

Tool Details

Target Details

Application Details

References

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

Tool name: Venus-A2AR vector
Alternate name: A2AR
Research fields: Cell biology;Neurobiology
Tool sub type: pcDNA 3.1
Bacterial resistance: Ampicillin
Selectable markers: Neomycin
Description: Venus-A2AR construct was generated with the In-fusion HD Cloning Kit (Clontech Takara, USA). Venus and A2AR fragments were produced by using, respectively, the pair of primers 5'-GTTTAAACTTAAGCTTATGGTGAGCAAGGGCGAG-3' and 5'-GCTGCCCATGGTGGCCTTGTACAGCTCGTCCATG-3', and the pair of primers 5'-GCCACCATGGGCAGCAGC-3' and 5'-AAACGGGCCCTCTAGATCAGCTGGGGGCGAACTC-3'. PCR fragments were cloned into the vector pcDNA3.1(ÄËĂÂĂÂ+ÄËĂÂĂÂ) linearized with HindIII and XbaI, and the resulting construct was verified by DNA sequencing (GATC Biotech, Germany).
Additional notes: A construct encoding a Venus-A2AR fusion protein to explore the synaptic function of A2AR

Target Details

Target: Adenosine A2A receptor

Application Details

Application notes: Venus-A2AR construct was generated with the In-fusion HD Cloning Kit (Clontech Takara, USA). Venus and A2AR fragments were produced by using, respectively, the pair of primers 5'-GTTTAAACTTAAGCTTATGGTGAGCAAGGGCGAG-3' and 5'-GCTGCCCATGGTGGCCTTGTACAGCTCGTCCATG-3', and the pair of primers 5'-GCCACCATGGGCAGCAGC-3' and 5'-AAACGGGCCCTCTAGATCAGCTGGGGGCGAACTC-3'. PCR fragments were cloned into the vector pcDNA3.1(â€‰+â€‰) linearized with HindIII and XbaI, and the resulting construct was verified by DNA sequencing (GATC Biotech, Germany).

References

Temido-Ferreira et al. 2020. Mol Psychiatry. 25(8):1876-1900. PMID: 29950682.