#158344

pUC57-2A vector

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Cat. #158344

pUC57-2A vector

Cat. #: 158344

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This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Inventor: Dr Claudio Franco

Institute: Instituto de Medicina Molecular JoĂŁo Lobo Antunes

Tool Details
Application Details
Related Tools
References

Tool Details

*FOR RESEARCH USE ONLY

  • Tool name: pUC57-2A vector
  • Research fields: Cell biology
  • Description: The two reporter sequences (for GTS-mCherry and H2B-GFP) are linked by the 2A self-cleaving peptide to allow expression of stoichiometric amounts of both fluorescent reporters. The 2A peptide-linked multicistronic vectors can be used to express multiple proteins from a single open reading frame (ORF). The small 2A peptide sequence, when cloned between genes, allows for efficient, stoichiometric production of discrete protein products within a single vector through a novel "cleavage" event within the 2A peptide sequence. 2A peptides are between 18-22 amino acids in length and have divergent amino-terminal sequences, (minimising the chance for homologous recombination. For more information see reference PMID: 22301656

Application Details

  • Application notes: The two reporter sequences (for GTS-mCherry and H2B-GFP) are linked by the 2A self-cleaving peptide to allow expression of stoichiometric amounts of both fluorescent reporters. The 2A peptide-linked multicistronic vectors can be used to express multiple proteins from a single open reading frame (ORF). The small 2A peptide sequence, when cloned between genes, allows for efficient, stoichiometric production of discrete protein products within a single vector through a novel "cleavage" event within the 2A peptide sequence. 2A peptides are between 18-22 amino acids in length and have divergent amino-terminal sequences, (minimising the chance for homologous recombination. For more information see reference PMID: 22301656

Related Tools

  • Related tools: pUC57-GNrep vector ; pME-GNrep vector

References

  • Barbacena et al. 2019. Genesis. 57(6):e23299. PMID: 30990965.
  • Szymczak-Workman et al. 2012. Cold Spring Harb Protoc. 2012(2):199-204. PMID: 22301656.