pBABE-hygro Vector

High-titer retroviral empty backbone pBABE vector, ready for cloning. pBABE-hygro enables stable gene delivery and expression in mammalian cells with MO-MuLV LTR-driven expression for reliable, long-term transgene production. Features Hygromycin…

Contributors

Inventor Institute
Hartmut Land, Jay Morgenstern Cancer Research UK London Research Institute: Lincoln's Inn Fields
SKU: 162284
Product description: High-titer retroviral empty backbone pBABE vector, ready for cloning. pBABE-hygro enables stable gene delivery and expression in mammalian cells with MO-MuLV LTR-driven expression for reliable, long-term transgene production. Features Hygromycin B selection useful for sequential selection studies. pBABE-hygro is a part of the established pBABE vector system, developed by Morgenstern and Land 1990. Key features include: high viral titers for efficient transduction, stable long-term gene expression via LTR promoter and helper-free design which minimises contamination risk.
Additional notes: The pBABE vectors prove useful in experiments where highest frequencies of gene transfer, or concomitant expression of several different genes within a single cell are required with minimal risk of helper virus contamination.
Cat. #: 162284
Unit size: Provided as 50-100ng cloning grade DNA on Whatman filter paper
Research Fields: Genetics
Total vector size: 5558
Backbone size without insert: 5558
Bacterial resistance: Ampicillin
Selectable markers: Hygromycin
Primary citation: Morgenstern et al. 1990. Nucleic Acids Res. 18(12):3587-96. PMID: 2194165.
Shipping conditions: Provided as 50-100ng cloning grade DNA on Whatman filter paper
Plasmid amplification details: DH5alpha 37° C
Plasmid copy number: High copy

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