dtUD1 vector

Affinity-tagged small ubiquitin related modifier (SUMO) fusion proteins generated by the cloning vectors: pETHSUL, pETS2SUL, pASHSUL, or pASS2SUL are treated with recombinant SUMO protease, generated from the pdtUD1 vector, to cleave SUMO and yield the mature target protein. Instructions for vector expression, purification, and use are outlined in: Weeks, S. D., Drinker, M., & Loll, […]

Contributors

Inventor Institute
Dr. Patrick Loll Drexel University
Cat. #: 156511
Tool sub type: pETSU vector containing an untagged yeast SUMO sequence
Research Fields: Other
Target: A dual tagged clone of the catalytic domain (dtUD1) of the <i> S. cerevisiae </i> SUMO hydrolase. The recombinant protease has an amino terminal Strep-II tag and a carboxy terminal His6 tag.
Bacterial resistance: Ampicillin
Product description: Affinity-tagged small ubiquitin related modifier (SUMO) fusion proteins generated by the cloning vectors: pETHSUL, pETS2SUL, pASHSUL, or pASS2SUL are treated with recombinant SUMO protease, generated from the pdtUD1 vector, to cleave SUMO and yield the mature target protein. Instructions for vector expression, purification, and use are outlined in: Weeks, S. D., Drinker, M., & Loll, P. J. (2007). Ligation independent cloning vectors for expression of SUMO fusions. Protein expression and purification, 53(1), 40–50. doi:10.1016/j.pep.2006.12.006 Protein Purification; Protein Biochemistry
Additional notes: The pdtUD1 vector allows the production of recombinant UD1 domain of the <i> S. cerevisiae </i> Ulp1 protein (small ubiquitin related modifier (SUMO) protease). The UD1 domain retains the full SUMO-specific proteolytic activity. The recombinant protease has an amino terminal Strep-II tag and a carboxy terminal His6 tag to allow for easy purification of the recombinant protease. The dual tags also allow the protease to be removed after cleavage of the SUMO fusion protein generated by the cloning vectors: pETHSUL, pETS2SUL, pASHSUL, or pASS2SUL, by using subtractive affinity chromatography.
Application notes: Affinity-tagged small ubiquitin related modifier (SUMO) fusion proteins generated by the cloning vectors: pETHSUL, pETS2SUL, pASHSUL, or pASS2SUL are treated with recombinant SUMO protease, generated from the pdtUD1 vector, to cleave SUMO and yield the mature target protein. Instructions for vector expression, purification, and use are outlined in: Weeks, S. D., Drinker, M., & Loll, P. J. (2007). Ligation independent cloning vectors for expression of SUMO fusions. Protein expression and purification, 53(1), 40–50. doi:10.1016/j.pep.2006.12.006 Protein Purification; Protein Biochemistry
References:

Weeks et al. 2007. Protein Expr Purif. 53(1):40-50. PMID: 17251035.

Related Tools for Vectors

Cat. # Tool Name
152050 pJEK21 Gamma-Synuclein Vector Key Info View Tool
152053 pKH2 Beta-Synuclein P123H Vector Key Info View Tool
153287 pCMV-iNOS Vector Key Info View Tool
153288 phOC-iNOS Vector Key Info View Tool
153546 pHyg-AID(1-114)-8myc Plasmid Key Info View Tool

Tool enquiry

Please ensure you use your organisation email address rather than personal where possible, as this helps us locate your organisation in our system faster.

Please note we may take up to three days to respond to your enquiry.

CancerTools.org uses the contact information provided to respond to you about our research tools and service. For more information please review our privacy policy.