HCI-013 breast cancer PDX

HCI-013 PDX: One of many Patient Derived Xenografts providing models for breast cancer including drug-resistant, metastatic tumours, and HER2+ tumours.

Contributors

Inventor Institute
Alana L Welm, Yi-Chun Lin, Yoko Sakata DeRose The University of Utah Research Foundation
SKU: 162081
Product description: HCI-013 PDX: One of many Patient Derived Xenografts providing models for breast cancer including drug-resistant, metastatic tumours, and HER2+ tumours.
Cat. #: 162081
Cancer types: Breast cancer
Cancer Subtype: Infiltrating Lobular Carcinoma
Gender: Female
Cancer Stage or Grade: Stage IV
Biopsy Site: Pleural Effusion Fluid
Patient ethnicity: Caucasian
Treatment History: Pretreated: Patient had undergone radiation therapy to bone other than spine (2007) and spine (2010) and had systemic treatment of leuprolide 2003-2008; letrozole 2003-2007; zolendronic acid 2003-2010; tamoxifen 2007-2008; exemestane 2008; cyclophosphamide, 2008; 5-fluorouracil, methotrexate 2008; capecitabine 2008-2009; paclitaxel 2009; nab-paclitaxel 2009; doxorubicin 2009-2010; carboplatin, gemcitabine 2010 prior to sample collection
Primary citation: Guillen, et al. 2022. Nature Cancer. Feb; 3(2):232-250. PMID: 35221336
Initial handling information: Implant into the cleared inguinal mammary fat pad of female Immune-compromised mice (NOD.SCID). Time to grow to 2cm: 3-6 months. Positive ER status
Additional notes: Additional Information on PDX establishment: https://www.nature.com/articles/s43018-022-00337-6/figures/9
Mice Passaged: Yes
Engraftment Site: Cleared mammary fat pad
Sample Type: Suspension in Matrigel
Host Strain: Immunocompromised mice NOD scid gamma (NSG) Jackson Laboratory 5557; NOD/scid, Jackson Laboratory 1303 or NOD rag gamma (NRG), Jackson Laboratory 7799
Histology: PAM50 subtype Luminal B
Production details: Fresh or thawed human breast tumour fragments were implanted into the cleared inguinal mammary fat pad of female Immune-compromised mice. For bone metastasis samples, bone fragments were coimplanted. For liquid specimens, pleural effusion, or ascites fluid, 1-2 milion cells were injected into cleared mammary fat pads in Matrigel. For ER+ tumours, mice were dosed with E2 beeswax pellets and given supplemental E2 via drinking water. When tumours reached 1-2 cm in diameter, tumours were aseptically collected and reimplanted into new m ice or banked. Estrogen-independent ER+ breast PDX models were generated when ER+ PDX tumours were transplated into overiectomized mice without E2 supplementation.
References:

Tufail, et al. 2024. Journal of Translational Med. Jan 3:22(1):15. PMID: 38172946

Bhattacharya, et al. 2023. Journal of Experimental & Clinical Cancer Research. Dec 16:42(1):343. PMID: 38102637

Prekovic, et al. 2023. EMBO Molecular Medicine. Dec 7:15(12):e17737. PMID: 37902007

Daneshdoust, et al. 2023. Cells. Sep 30:12(19):2338. PMID: 37830602

Wang, et al. 2023. Cell Bioscience. Dec 1:13(1):224. PMID: 38041134

Guillen, et al. 2022. Nature Cancer. Feb 3(2):232-250. PMID: 35221336

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