HCI-002 breast cancer PDX

Breast cancer patient-derived xenograft (PDX) model, HCI-002, one of a collection of 53 models spanning aggressive, metastatic, and treatment-resistant subtypes, that recapitulates key characteristics of original patient tumour. This model was generated from a sample of breast tumour from a Caucasian female, 61 years old with a primary diagnosis of infiltrating ductal carcinoma (IDC). The patient had been a smoker for 40 years, had not undergone radiation therapy or systemic treatment prior to collection, and had clinical metastasis to lymph node, bone, and lung.

Contributors

Inventor Institute
Alana L Welm, Yi-Chun Lin, Yoko Sakata DeRose The University of Utah Research Foundation
Cat. #: 162070
Cancer types: Breast cancer
Cancer Subtype: Infiltrating Ductal Carcinoma
Gender: Female
Cancer Stage or Grade: Stage IIIa, grade 3
Biopsy Site: Breast (primary)
Patient ethnicity: Caucasian
Primary citation: Guillen, et al. 2022. Nature Cancer. Feb; 3(2):232-250. PMID: 35221336
Product description: HCI-002 PDX: Sample from breast tumour of Caucasian female, 61 years old with a primary diagnosis of IDC; 2009 and had been a smoker for 40 years.
Initial handling information: Implant into the cleared inguinal mammary fat pad of female Immune-compromised mice (NOD.SCID). Time to grow to 2cm: 1 month
Additional notes: Additional Information on PDX establishment: https://www.nature.com/articles/s43018-022-00337-6/figures/9
Mice Passaged: Yes
Engraftment Site: Cleared mammary fat pad
Sample Type: Tumour fragments
Host Strain: Immunocompromised mice NOD scid gamma (NSG) Jackson Laboratory 5557; NOD/scid, Jackson Laboratory 1303 or NOD rag gamma (NRG), Jackson Laboratory 7799
Histology: PAM50 subtype Basal-like
Production details: Fresh or thawed human breast tumour fragments were implanted into the cleared inguinal mammary fat pad of female Immune-compromised mice. For bone metastasis samples, bone fragments were coimplanted. For liquid specimens, pleural effusion, or ascites fluid, 1-2 milion cells were injected into cleared mammary fat pads in Matrigel. For ER+ tumours, mice were dosed with E2 beeswax pellets and given supplemental E2 via drinking water. When tumours reached 1-2 cm in diameter, tumours were aseptically collected and reimplanted into new m ice or banked. Estrogen-independent ER+ breast PDX models were generated when ER+ PDX tumours were transplated into overiectomized mice without E2 supplementation.
References:

Tufail, et al. 2024. Journal of Translational Med. Jan 3:22(1):15. PMID: 38172946

Bhattacharya, et al. 2023. Journal of Experimental & Clinical Cancer Research. Dec 16:42(1):343. PMID: 38102637

Prekovic, et al. 2023. EMBO Molecular Medicine. Dec 7:15(12):e17737. PMID: 37902007

Daneshdoust, et al. 2023. Cells. Sep 30:12(19):2338. PMID: 37830602

Wang, et al. 2023. Cell Bioscience. Dec 1:13(1):224. PMID: 38041134

Guillen, et al. 2022. Nature Cancer. Feb 3(2):232-250. PMID: 35221336

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