US/VOT-E36 Cell Line
A mouse inner ear epithelial progenitor model used to investigate sensory cell differentiation, inner ear development, and developmental signalling pathways. Save 30% on this cell line this autumn. Use code ACA26CELL at checkout.
Contributors
| Inventor | Institute |
|---|---|
| Matthew C Holley | University of Sheffield |
| SKU: | 153625 |
|---|---|
| Product description: | Derived from epithelial cells from the ventral region of the otocyst at embryonic day E10.5 (plug in mouse designated E0.5 and birth at E18-19). At this stage the sensory epithelia have not differentiated and the epithelium is competent to form most of the cells within the cochlear duct, including primary sensory neurons. VOT-E36 has been characterised extensively by timed expression under differentiating conditions in vitro of a combination of gene and protein markers for epithelial cells and for inner ear sensory cells. It has also been screened with Affymetrix mouse micro-arrays. It forms cells of various cochlear phenotypes under differentiating conditions in vitro and despite being derived from an earlier developmental stage, it produces more advanced states of sensory cell differentiation than occurs with UB/OC-1, UB/OC-2. The cells are also available with a stably incorporated EGFP reporter that has been tested both in vitro and in vivo. |
| Alternate name: | US/VOT-E36; Ventral Otocyst-Epithelial cell line number 36; VOT-E36; University of Sheffield/Ventral OTocyst-Epithelial 36 |
| CRISPR: | No |
| Conditional: | Yes |
| Conditional description: | The cells were derived from ImmortomiceTM (Jat et al 1991 Proc. Nat. Acad. Sci. USA 88, 5096-5100) carrying a stable insertion of the conditional immortalising gene H-2Kb-tsA58, which describes a temperature-sensitive variant of the SV40 immortalising gene that encodes the large tumour antigen under the control of the ?interferon-sensitive MHC Class 1 promoter. The cells proliferating culturing conditions: 33°C with ?IFN; and the cells differentiating culturing conditions: 39… |
| Production details: | Homozygous male Immortomice (originally derived from injected oocytes of CBA/Ca x C57BL/10 mice) were time-mated with wild-type C57Bl/6 female mice to produce heterozygous offspring. Animals were killed by cervical dislocation, in accordance with UK Home Office regulations. Otocysts were removed from E10 embryos under sterile conditions and then dissected to isolate the ventral region. Further selection of ventral otocyst explants was based on expression of the immortalizing gene (condition… |
| Cellosaurus ID: | CVCL_VC36 |
| Model description: | Conditionally immortalized cell line |
| Disease: | Deafness |
| Cat. #: | 153625 |
|---|---|
| Tool sub type: | Continuous |
| Unit size: | 1×10^6 cells / vial |
| Research Fields: | Developmental biology; Drug development; Genetics |
| Organism: | Mouse |
| Tissue: | Embryonic |
| Model: | Transgenic |
| Morphology: | Epithelial |
| Growth properties: | Adherent |
| Primary citation: | Lawoko-Kerali et al. 2004. Dev Dyn. 231(4):801-14. PMID: 15499550 |
| Format: | Frozen |
|---|---|
| Storage conditions: | Liquid Nitrogen |
| Shipping conditions: | Dry ice |
| Growth medium: | MEM with 10% FCS, 50 Units/ml y-IFN, L-glutamine |
| Temperature: | 33° C |
| Storage medium: | Cytiva HyCloneTM FetalClone II Serum (U.S.) Thermo Fisher |
| Mycoplasma free: | Yes |
| Biosafety level: | 1 |
| References: |
Holley et al. 2007. Hear Res. 227(1-2):32-40. PMID: 16797894 Helyer et al. 2007. Eur J Neurosci. 25(4):957-73. PMID: 17331193 Liu et al. 2006. Otol Neurotol. 27(3):414-21. PMID: 16639283 Lawoko-Kerali et al. 2004. Dev Dyn. 231(4):801-14. PMID: 15499550 |
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