UB-UE1 Cell Line

An immortalised mouse vestibular epithelial cell model used to study inner ear development, hair cell regeneration, and sensory epithelial differentiation. Save 30% on this cell line this summer. Use code SUM26CELL at checkout.

Contributors

Inventor Institute
Matthew C Holley University of Bristol; University of Sheffield
SKU: 153624
Product description: Derived from vestibular epithelium (utricular macula) using thermolysin treatment and dissection to ensure origin from sensory epithelial supporting cells at post-natal day P2. At this stage the supporting cells are normally post-mitotic but retain the ability to differentiate as sensory hair cells and or supporting cells. UB/UE-1 was characterised by timed expression under differentiating conditions in vitro of a combination of gene and protein markers for epithelial cells and for inner ear sensory cells. These include Cytokeratin, Vimentin, Myosin VIIa, Myosin VI, Brn3c, alpha9AChR and a combination of functional ion channels (see references). Screened with Affymetrix mouse Micro-arrays. The cells differentiate neonatal stage hair cell and supporting cell phenotypes under differentiating conditions in vitro
Alternate name: UB-UE1; UB/UE-1; University of Bristol/Utricular Epithelium-1; Utricular Epithelium cell line number 1
Conditional: Yes
Conditional description: Cells derived from C57 Bl6 mice carrying a stable insertion of the conditional immortalising gene H-2Kb-tsA58, which describes a temperature-sensitive variant of the SV40 immortalising gene that encodes the large tumour antigen under the control of the gamma interferon-sensitive MHC Class 1 promoter. The transgenic mouse is called the ImmortomouseTM (Jat et al 1991 Proc. Nat. Acad. Sci. USA 88, 5096-5100)
Cellosaurus ID: CVCL_D807
Parental cell line: Pure utricular sensory epithelia of 2-day old immortomouse pups
Disease: Hearing loss
Cat. #: 153624
Unit size: 1×10^6 cells / vial
Research Fields: Developmental biology;Drug development;Genetics
Organism: Mouse
Model: Immortalised Line
Primary citation: Lawlor et al. 1999. J Neurosci. 19(21):9445-58. PMID: 10531448
Format: Frozen
Storage conditions: Liquid Nitrogen
Shipping conditions: Dry ice
Growth medium: MEM with 10% FCS, 50Units/ml y-IFN, L-glutamine,
Temperature: 33° C
Mycoplasma free: Yes
Biosafety level: 1
References:

Hackett et al. 2002. Exp Cell Res. 278(1):19-30. PMID: 12126954

Lawlor et al. 1999. J Neurosci. 19(21):9445-58. PMID: 10531448

Images

View Gallery

Related Tools for Cell lines

Cat. # Tool Name
151558 MEF Trex1 KO Cell Line Key Info View Tool
151559 MEF UNG KO Cell Line Key Info View Tool
151448 CMT 167 Cell Line Key Info View Tool
151449 JIM1 Cell Line Key Info View Tool
151655 FLYA-13 Cell Line Key Info View Tool

Related News

UM-UC-10 Cell Line. Image courtesy of the European Collection of Authenticated Cell Cultures (ECACC), UK.
Cell Lines

Popular cell lines now available only from CancerTools.org

Read More
Cell Lines

New cell lines: murine melanocyte and melanoblast cell lines from Dr. Elena Sviderskaya

Read More
Image Reference: 2x PTEN -/- mouse prostate cancer cell lines (MuCap system). Histology staining of syngraft tumours (Van Duijn et al., 2018).
Cell Lines

38 new prostate cancer cell lines

Read More

Tool enquiry

Please ensure you use your organisation email address rather than personal where possible, as this helps us locate your organisation in our system faster.

Please note we may take up to three days to respond to your enquiry.

CancerTools.org uses the contact information provided to respond to you about our research tools and service. For more information please review our privacy policy.