TOV-1946 cell line

Solid-tumour-derived serous ovarian cancer cell line matched to ascites-derived OV-1946 from the same chemotherapy-naïve patient

Contributors

Inventor Institute
Anne-Marie Mes-Masson and Diane Provencher Centre Hospitalier de L’université de Montréal
SKU: 161770
Product description: TOV-1946 was established from the solid tumour of a chemotherapy-naïve patient with stage IIIC, grade 3 serous papillary ovarian carcinoma and is matched to OV-1946, derived from ascites from the same patient. The two models carry the same somatic TP53 mutation but also show distinct chromosomal features, making them useful for comparing solid-tumour- and ascites-derived disease while controlling for patient background. Both TOV-1946 and OV-1946 were shown to form spheroids and tumours in SCID mice, and all three lines characterised in the defining study showed anchorage-independent growth.
Alternate name: TOV-1946
Gender: Female
CRISPR: No
Production details: Established using the Scrape method where tumor tissue was scraped into a 100mm plate with complete OSE medium and maintained for 40 days with medium replaced weekly. After 40 days, 80% confluence was attained and cells were divided intwo 2 petri dishes and further divided in a proportion of 2:3 once a week for the first 15 passages and 1:2 twice a week until passage 70. Subsequently, cells were divded in a proportion of 1:5 twice a week.
Additional notes: Patient 1946 had no known familial history of cancer and passed from post-operative complications
Receptors of note: No
Related tools: 161771
Cat. #: 161770
Cancer types: Ovarian cancer
Research Fields: Cancer
Organism: Human
Tissue: Derived from poorly differentiated serous solid tumors
Gender: Female
Morphology: Able to form loose aggregate of cells. Expressed Krt7
Growth properties: Adherent
Primary citation: Ouellet et al. 2008. BMC Cancer. (8):152. PMID: 18507860
Shipping conditions: Dry Ice
Growth medium: OSE medium contains 10% FBS, 0.5ug/mL amphotericin B and 50 ug/mL gentamicin
Atmosphere: Hypoxic condition of 5%O2 and 5%CO2
References:

Sauriol et al. 2023. Sci Rep. 27

13(1):3334. PMID: 36849518 Sevinyan et al. 2022. Cancers (Basel). 16

14(22):5628. PMID: 36428724 Brodeur et al. 2021. Sci Rep. 14

11(1):18183. PMID: 34521878 Communal et al. 2021. Int J Mol Sci. 18

22(10):5325. PMID: 34070214 Patra et al. 2020 PLoS One. 31

15(12):e0244549. PMID: 33382759

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161771 OV-1946 cell line Key Info View Tool

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