SW480 EGFP-lamin A cell line clone 12

Duke’s stage C/Broders’ grade 3-4 colorectal cancer cell line stably transfected with EGFP tagged lamin A.

Contributors

Inventor Institute
Stefan Przyborski University of Durham
SKU: 162198
Product description: Colon cancer cell line with low endogenous lamin A protein expression, rescued by stable transfection of EGFP-lamin A. Suitable for studying the effect of the presence of lamin A on cancer cell properties.
Alternate name: SW480 GFP-lamin A clone 11
Receptors of note: No
Parental cell line: SW480
Cat. #: 162198
Organism: Human
Tissue: Colon
Model: Genetically modified cell line
Morphology: Epithelial
Growth properties: Adherent
Initial handling information: Continue to store at ?-70deg C. To bring up cells, defrost quickly in 37?C water bath and seed in petri dish/flask. Once cells have adhered to culture surface, change media to DMSO-free media.
Format: Frozen
Storage conditions: Liquid Nitrogen
Shipping conditions: Dry ice
Growth medium: L15 (Leibovitz) medium with 2mM L-Glutamine, 10% FBS and 100units/ml penicillin / 100ug/ml streptomycin
Subculture routine: Passage at 70-80% confluencey in the presence of 0.25% trypsin in 0.5mM EDTA/PBS. Incubate at 37?C, 5% Co2, humidified for 2 minutes. Split 1:4.
Atmosphere: Without CO2
Storage medium: L15 (Leibovitz) medium with 2mM L-Glutamine, 10% FBS and 10% DMSO
Recommended controls: Same cell line stably transfected with EGFP not tagged to any other DNA

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