OV-4485 cell line

Ascites-derived high-grade serous ovarian cancer cell line carrying the pathogenic BRCA1 c.4485-1G>T (IVS14-1G>T) splice-site variant

Contributors

Inventor Institute
Anne-Marie Mes-Masson and Diane Provencher Centre Hospitalier de L’université de Montréal
SKU: 161788
Product description: OV-4485 is a high-grade serous ovarian cancer cell line established from ascites. The patient harboured the germline BRCA1 c.4485-1G>T (IVS14-1G>T) splice-site variant, which was also identified in the cell line in the homozygous state. The defining study showed abnormal BRCA1 splicing and no evident BRCA1 protein band in OV-4485, consistent with loss of BRCA1 function. Subsequent clinical variant-classification work has classified BRCA1 c.4485-1G>T as pathogenic. OV-4485 therefore provides a characterised BRCA1-deficient HGSOC model for investigating BRCA-associated tumour biology and for comparison with BRCA2-altered models such as OV-4453.
Alternate name: OV-4485
Gender: Female
CRISPR: No
Production details: Established from the cellular fraction of ascites collected by centrifugation. The cell lines were then maintained for 40 days in OSE with medium replaced weekly.
Additional notes: Patient 4485 was age 55 at diagnosis, BRCA hereditary status was BRCA1(+), and no prior personal history of cancer. Patient received Carboplatin/taxol, interval sugery, and was later in a clinical trial: maintenance sorafenib vs placebo trial.
Cellosaurus ID: CVCL_9T21
Receptors of note: No
Related tools: 161787
Cat. #: 161788
Research Fields: Cancer
Organism: Human
Tissue: Derived from ascites
Gender: Female
Morphology: Formed loosely compact spheroids with irregular margins
Growth properties: Adherent
Primary citation: Fleury et al. 2015. Genes & Cancer. 6(9-10):378-398. PMID: 26622941
Shipping conditions: Dry Ice
Growth medium: OSE medium contains 10% FBS, 0.5ug/mL amphotericin B and 50 ug/mL gentamicin
Atmosphere: Low oxygen conditions of 7% O2 and 5% CO2
References:

Vias et al. 2023. Elife. 11 12:e83867. PMID: 37166279

Sevinyan et al. 2022. Cancers (Basel). 16 14(22):5628. PMID: 36428724

Asare-Werehene et al. 2023. Cancers (Basel). 30 15(9):2566. PMID: 37174032

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