OV-4453 cell line

Ascites-derived high-grade serous ovarian cancer cell line carrying a BRCA2 p.Glu1953Ter mutation, established before ovarian cancer chemotherapy

Contributors

Inventor Institute
Anne-Marie Mes-Masson and Diane Provencher Centre Hospitalier de L’université de Montréal
SKU: 161787
Product description: OV-4453 is a high-grade serous ovarian cancer cell line established from ascites collected at diagnosis before ovarian cancer chemotherapy. The patient had previously received radiotherapy and hormonotherapy for breast cancer five years before the ovarian cancer diagnosis, an important consideration when interpreting the model as treatment-naïve for ovarian cancer. The cell line carries a BRCA2 c.5857G>T (p.Glu1953Ter) mutation documented in the defining study, and provides a characterised BRCA2-altered HGSOC model for investigating BRCA-associated tumour biology and for comparison with ovarian cancer models carrying different BRCA backgrounds.
Alternate name: OV-4453
Gender: Female
CRISPR: No
Production details: Established from the cellular fraction of ascites collected by centrifugation. The cell lines were then maintained for 40 days in OSE with medium replaced weekly.
Additional notes: Patient 4453 was age 70 at diagnosis, BRCA hereditary status was BRCA2(+), and had previous history of breast cancer. Treatment for ovarian cancer included Carboplatin/taxol and interval surgery. Year of sampling was 2009.
Cellosaurus ID: CVCL_9T20
Receptors of note: No
Related tools: 161788
Cat. #: 161787
Cancer types: Ovarian cancer
Research Fields: Cancer
Organism: Human
Tissue: Derived from ascites from patient with high grade serous carcinoma
Gender: Female
Morphology: Formed numerous individual small aggregates
Growth properties: Adherent
Primary citation: Fleury et al. 2015. Genes & Cancer. 6(9-10):378-398. PMID: 26622941
Growth medium: OSE medium contains 10% FBS, 0.5ug/mL amphotericin B and 50 ug/mL gentamicin
Atmosphere: Low oxygen conditions of 7% O2 and 5% CO2
References:

Vias et al. 2023. Elife. 11,12:e83867. PMID: 37166279 Sevinyan et al. 2022. Cancers (Basel). 16,14(22):5628. PMID: 36428724

Asare-Werehene et al. 2023. Cancers (Basel). 30

15(9):2566. PMID: 37174032

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