NBF0LN7-1120BL Cell Line
Seventh generation cell line derived from an ipsilateral brachial lymph node metastasis of a sixth-generation line
Contributors
| Inventor | Institute |
|---|---|
| Nathan E. Reticker-Flynn | Stanford University |
| Cat. #: | 162232 |
|---|---|
| Cancer types: | Skin cancer |
| Organism: | Mouse |
| Tissue: | Skin (lymph node metastasis) |
| Gender: | Female |
| Model: | Tumorigenic cell line; syngeneic |
| Morphology: | Mixed morphology that is predominantly spindle-shaped |
| Product description: | Seventh generation cell line derived from an ipsilateral brachial lymph node metastasis of a sixth-generation line |
|---|---|
| Gender: | Female |
| CRISPR: | No |
| Production details: | Line was isolated from a brachial lymph node metastasis after roughly one month of growth of a LN6 tumor |
| Parental cell line: | B16-F0 |
| Model description: | A luciferase transgene was integrated into the B16-F0 parental line, but is no longer expressed in LN metastatic progeny. WES suggests the presence of Braf and Pten mutations. |
| Disease: | Melanoma |
| Storage conditions: | Liquid Nitrogen |
|---|---|
| Shipping conditions: | Dry Ice |
| Growth medium: | DMEM (high glucose) + 10% FBS + 4mM L-glutamine (or GlutaMAX) + 1% Pen/Strep (optional) |
| Subculture routine: | See product datasheet |
| Temperature: | 37C |
| Atmosphere: | 5% CO₂ |
| Storage medium: | DMEM, 50% FBS, 10% DMSO |
| Mycoplasma free: | Yes |
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