Human AGS NOD1 KO (Clone 41H8) Cell Line

NOD1 responds to Helicobacter pylori infection in a Type IV secretion system-dependent manner. H. pylori causes chronic inflammation, a key precursor to gastric adenocarcinoma. AGS cells deficient in NOD1 (as generated by CRISPR) can be used to model gastric adenocarcinoma in the context of various pathogens.

Contributors

Inventor Institute
Richard Ferrero ; Le Son Tran Hudson Institute of Medical Research
SKU: 153734
Product description: NOD1 responds to Helicobacter pylori infection in a Type IV secretion system-dependent manner. H. pylori causes chronic inflammation, a key precursor to gastric adenocarcinoma. AGS cells deficient in NOD1 (as generated by CRISPR) can be used to model gastric adenocarcinoma in the context of various pathogens. NOD1 regulates host epithelial cell responses. Upregulates antimicrobial peptides of the beta-defensin (hBD) family secreted by epithelial cells.
Alternate name: Human nucleotide-binding oligomerization domain-containing protein 1, NOD1
Conditional: No
Production details: CRISPR/Cas9 gene editing was used to generate NOD1 knock out clones. AGS cells were transfected with vectors expressing â?‚€?‚ÂœCas9 nickaseâ?‚€?‚ nuclease and pairs of guide RNAs (gRNAs) specific for the NOD1 gene. Clone 41H8 was confirmed as having a 31 base pair deletion within the coding regions of the caspase activation and recruitment domain (CARD) of NOD1. Recommended Freezing Composition: 90% (v/v) FCS – 10% (v/v) DMSO
Additional notes: Recommended Freezing Composition: 90% (v/v) FCS – 10% (v/v) DMSO CRISPR edited Human AGS cells. Cancer Research Technology Limited (trading research tools as Ximbio) has been granted a non-exclusive license to the CRISPR-Cas9 technology by ERS Genomics Ltd under the patent rights listed here. This license from ERS Genomics Ltd allows Ximbio to develop and commercialise CR…
Parental cell line: AGS
Disease: Cancer
Cat. #: 153734
Tool sub type: Continuous
Unit size: 1×10^6 cells / vial
Cancer types: Gastric cancer
Research Fields: Cancer; Cell signaling and signal transduction; Immunology
Organism: Human
Tissue: Gastric
Model: Knock-Out
Growth properties: Epithelial-like morphology
Cancer Types In Detail: Gastric Adenocarcinoma;Helicobacter pylori infection
Format: Frozen
Storage conditions: Liquid Nitrogen
Shipping conditions: Dry ice
Growth medium: RPMI with: 10% foetal calf serum; 1% penicillin-streptomycin; and 1% L-glutamine. Subculture cells every 2-3 days in ratios of 1:2-1:4 in T25 or T75 flasks.
Recommended controls: NOD1 Ligand: C12-iE-DAP (10 ng/ml), Human AGS Control Cell Line
Mycoplasma free: Yes
Biosafety level: 1
References:

Tran et al. 2018. Cell Microbiol. 20(5):e12826. PMID: 29392836.

NOD1 is required for Helicobacter pylori induction of IL-33 responses in gastric epithelial cells.

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