Human AGS NOD1 KD (AGS cl.1) Cell Line

These cell lines have for example been used to study NOD1 responds to Helicobacter pylori infection. H. pylori causes chronic inflammation, a key precursor to gastric adenocarcinoma. AGS cells deficient in NOD1 can be used to model gastric adenocarcinoma in the context of various pathogens.

Contributors

Inventor Institute
Richard Ferrero Hudson Institute of Medical Research
SKU: 153735
Product description: NOD1 regulates host epithelial cell responses. Upregulates antimicrobial peptides of the beta-defensin (hBD) family secreted by epithelial cells. NOD1 is an intracellular pattern recognition receptor that mediates innate and adaptive immunity. NOD1 specifically recognises molecules with a D-glutamyl-meso-diaminopimelic acid (iE-DAP) moiety, such as the Gram-negative bacterial cell wall component, peptidoglycan, which results in the activation of the NF-kB pathway and induction of cytokines via RIPK2 and IKK-gamma. Confers reactivity to bacterial liposaccharides (LPS) and confers immunity to viral and bacterial pathogens. These cell lines have for example been used to study NOD1 responds to Helicobacter pylori infection. H. pylori causes chronic inflammation, a key precursor to gastric adenocarcinoma. AGS cells deficient in NOD1 can be used to model gastric adenocarcinoma in the context of various pathogens.
Alternate name: Human nucleotide-binding oligomerization domain-containing protein 1, NOD1
Production details: Human AGS NOD1 Knock-down (KD) cells were generated by integration of an expression vector containing a small interference RNA (siRNA) directed to the gene encoding the CARD domain of the NOD1 gene. Recommended Freezing Composition: 90% (v/v) FCS – 10% (v/v) DMSO
Parental cell line: AGS
Disease: Cancer
Cat. #: 153735
Tool sub type: Continuous
Unit size: 1×10^6 cells / vial
Cancer types: Gastric cancer
Research Fields: Cancer; Cell signaling and signal transduction; Immunology
Organism: Human
Tissue: Gastric
Cancer Types In Detail: Gastric Adenocarcinoma;Helicobacter pylori infection
Format: Frozen
Shipping conditions: Dry ice
Growth medium: RPMI with: 10% foetal calf serum; 1% penicillin-streptomycin; and 1% L-glutamine. Subculture cells every 2-3 days in ratios of 1:2-1:4 in T25 or T75 flasks.
Storage medium: 90% (v/v) FCS – 10% (v/v) DMSO
Recommended controls: Human AGS Control (clone siEGFP)
Mycoplasma free: Yes
Biosafety level: 1
References:

Grubman et al. 2010. Cell Microbiol. 12(5):626-39. PMID: 20039881.

The innate immune molecule, NOD1, regulates direct killing of Helicobacter pylori by antimicrobial peptides.

Related Tools for Cell lines

Cat. # Tool Name
153734 Human AGS NOD1 KO (Clone 41H8) Cell Line Key Info View Tool

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