GFP-expressing D1-MSC cell line

Contributors

Inventor Institute
Jose Luis Pedraz ; Laura Saenz del Burgo ; JesĂ?Ÿs Ciriza University of the Basque Country (EHU)
SKU: 160552
Product description: Mesenchymal stem cells (MSCs) are multipotent stem cells found in bone marrow that are important for making and repairing skeletal tissues, such as cartilage, bone and the fat found in bone marrow. D1-MSC’s are a cell line of murine MSCs from bone marrow. D1-MSCs were transduced with the lentiviral construct pSIN-EF2-GFP-Pur, which codifies GFP from Aequorea coerulescens under the human elongation factor-1 alpha (EF-1 alpha) promoter, linked to puromycin resistance through an internal ribosome entry site (IRES) motif.
Conditional: No
Production details: Complementary DNA (cDNA) of GFP was cloned from the plasmid pAcGFP1-N1 containing GFP from Aequorea coerulescens and cloned on a lentiviral vector (pSIN) to give pSIN-EF2-GFP-Pur. This was used to transfect HEK293 cells from which the supernatant-containing lentivirus was harvested after 3 days. This supernatant was used to transduce D1-MSCs for 1 month after which GFP expression was confirmed by fluorescence microscopy. Lack of virus production by the now GFP-expressing D1-MSCs was confirmed…
Parental cell line: D1-MSC
Cat. #: 160552
Unit size: 1×10^6 cells / vial
Research Fields: Cell biology;Developmental biology
Organism: Mouse
Tissue: Bone Marrow
Model: Stem Cells
Growth properties: Adherent cells
Format: Frozen
Shipping conditions: Dry ice
Growth medium: DulbeccoÂ?‚´s Modified EagleÂ?‚´s Medium (DMEM) high glucose media supplemented with 10% heat-non-inactivated fetal calf serum and 1 unit/mL of penicillin-streptomycin and puromycin at 12.5 ÎĚ?Ÿg/mL.

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