GCGR E20 Cell line

The Glioma Cellular Genetics Resource (GCGR) is a panel of primary, patient-derived IDH-wildtype glioblastoma stem-like models, offering a biologically relevant system to capture inter-patient heterogeneity in tumour growth, behaviour, and molecular features.

Contributors

Inventor Institute
Gillian Morrison, Steven Pollard University of Edinburgh
Cat. #: 140061
Tool sub type: Primary
Cancer types: Brain cancer
Organism: Human
Tissue: Glioblastoma
Gender: Female
Model: Primary cell line
Morphology: Adherent monolayer with stem-like features (SOX2, NESTIN).
Growth properties: Adherent
Cancer Types In Detail: Glioblastoma
Product description: GCGR‑E20 is a primary, patient‑derived, IDH‑wildtype glioblastoma stem‑like cell line derived from human tumour tissue. The line grows as an adherent, laminin‑dependent monolayer, has a doubling time of approximately 159 hours, and forms tumours in vivo. Early‑passage transcriptomic profiling (P3) indicated a proneural‑like transcriptional state based on probability scoring. The line shows balanced development‑ and injury‑associated gene expression features with low enrichment for mesenchymal‑immune (MESImm) gene programmes.
Gender: Female
Production details: Derived from surgically resected primary glioblastoma tissue and expanded as an adherent monolayer under laminin supported condition
Additional notes: This cell line is a primary glioblastoma stem‑like model derived from a single patient tumour and exhibits intrinsic cellular heterogeneity, reflecting the diversity and plasticity typical of glioblastoma. Transcriptional state classifications were assessed at early passage (P3) and may change in culture
Format: Frozen
Growth medium: Serum‑free DMEM/HAMS‑F12 (“Complete + EFL”) supplemented with glucose, NEAA, B27, N2, BSA, beta‑mercaptoethanol, Pen/Strep, and recombinant EGF (10 ng/mL), FGF‑2 (10 ng/mL), plus laminin‑1 (2 ug/mL). Laminin is required for adherent growth.
Subculture routine: Passage at 80–90% confluence; split 1:4–1:6; detach using Accutase; avoid over‑dilution; replace medium weekly
Recommended controls: GCGR human neural stem cell lines
Biosafety level: 2
References:

Pollard SM et al. Cell Stem Cell 2009 PMID: 19497285.

Al Shboul S et al. Life Sci Alliance 2021 PMID: 34645618.

Purshouse K et al. eLife 2022 PMID:36476408.

Clements M et al. Nature 2025 PMID: 40836081.

Furqan M et al. Pharmaceutics 2025 PMID: 40430842.

Elliott R et al. A comprehensive pharmacological survey across heterogeneous patient‑derived GBM stem cell models. bioRxiv 2025.

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