CMT 64 Cell Line

A murine lung cancer cell line widely used for tumour growth, metastasis, and in vivo oncology studies. Available exclusively through CancerTools. Save 30% on this cell line this summer. Use code SUM26CELL at checkout.

Contributors

Inventor Institute
Peter Riddle Cancer Research UK, London Research Institute: Lincoln's Inn Fields
SKU: 151447
Product description: The tumourigenic and metastatic CMT 64 murine cell line is an in vivo mouse tumourigenesis system to study the growth characteristics and metastasis of mouse tumour lines (homoplastic with stable CMT 167 line); it demonstrates stable growth characteristics and morphology in culture and in lung metastasis induced after subcutaneous inoculation of mice. This demonstrates its consistency and reliability as a model for studying lung carcinoma progression and metastasis.
Alternate name: CMT-64; CMT.64; CMT64; C57 Mouse Tumor 64
Gender: Female
Production details: CMT 64 was isolated from a primary alveogenic lung carcinoma tumour in a C57BL/1CRF mouse. The cell line maintained a stable morphology and growth rate comparable to that observed in the original tumour tissue in vitro. Notably, CMT 64 retained similar characteristics in both primary tumour growth and in lung metastases following subcutaneous inoculation in mice.
Cellosaurus ID: CVCL_2406
Disease: Cancer
Cat. #: 151447
Provenance and Ownership: The CMT 64 Cell Line is owned by Cancer Research UK. CancerTools supplies the CMT 64 Cell Line under licence from Cancer Research UK and is the authorised source for its distribution. Sourcing CMT 64 Cell Line directly from the IP owner ensures authenticity, provenance and compliance with all applicable licensing and research use requirements.
Unit size: 1×10^6 cells / vial
Cancer types: Lung cancer
Research Fields: Cancer;Drug development
Organism: Mouse
Tissue: Lung
Gender: Female
Model: Cancer cell line
Growth properties: Adherent
Cancer Types In Detail: Lung carcinoma;Metastatic
Primary citation: Franks et al. 1976. Cancer Res. 36(3):1049-1055. PMID: 1253168.
Format: Frozen
Shipping conditions: Dry ice
Growth medium: DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculture routine: Split sub-confluent cultures (70-80%) 1:4 to 1:10 seeding at approximately 3 x 10^4 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Saturation density 1.2 x 10^5 cells/cm². Please also see detailed protocol within the Product Datasheet in the Documentation section below.
References:

Miyashita N et al. 2021. Sci Rep. 17:11(1):22380. PMID: 34789779

Rincon et al. 2017. Oncotarget. PMID: 28525366

Franks et al. 1976. Cancer Res. 36(3):1049-1055. PMID: 1253168.

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CMT 64 Cell Line. Image courtesy of the European Collection of Authenticated Cell Cultures (ECACC)

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