3.334 SC5/8 Cell Line

Hybrid between un-transformed mouse 3T3TK3 cells and normal rat kidney cells transformed with RSV. The hybrid cells express a non-transformed phenotype. The cells exhibit anchorage-independent growth at 35C but not at 39.5C, the non-permissive temperature and showed differences in microfilament bundle formation at both temperatures.

Contributors

Inventor Institute
Chris Marshall Cancer Research UK, London Research Institute: Lincoln's Inn Fields
Cat. #: 153249
Tool sub type: Continuous
Unit size: 1×10^6 cells / vial
Research Fields: Cell biology
Organism: Hybrid
Tissue: Fibroblast
Model: Tumour line
Growth properties: Adherent
Product description: Hybrid between un-transformed mouse 3T3TK3 cells and normal rat kidney cells transformed with RSV. The hybrid cells express a non-transformed phenotype. The cells exhibit anchorage-independent growth at 35C but not at 39.5C, the non-permissive temperature and showed differences in microfilament bundle formation at both temperatures.
Conditional: No
Additional notes: Rat x Mouse hybrid
Cellosaurus ID: CVCL_7237
Parental cell line: 3T3TK3 cells and normal rat kidney cells
Format: Frozen
Shipping conditions: Dry ice
Growth medium: Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4×10,000 cells/cm?‹Â› using 0.25% trypsin/EDTA; 35?‚°C; 5% CO2.Culture Medium: DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
References:

Marshall et al. 1980. Exp Cell Res. 127(2):373-84. PMID: 6247175.

Suppression of the transformed phenotype with retention of the viral 'src' gene in cell hybrids between Rous sarcoma virus-transformed rat cells and untransformed mouse cells.

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