Anti-Cdc20 [AR12.4]

Cdc20 (fizzy protein) exhibits protein kinase activity in vitro and exists in a complex with both cyclin B and a protein homologous to p13SUC1. Cdc2 kinase is the active subunit of the M phase promoting factor (MPF) and the M phase-specific Histone H1 kinase. The p34Cdc2/cyclin B complex is required for the G2 to M transition.

Contributors

Institute
European Institute of Oncology
SKU: 151310
Product description: Cdc20 (fizzy protein) exhibits protein kinase activity in vitro and exists in a complex with both cyclin B and a protein homologous to p13SUC1. Cdc2 kinase is the active subunit of the M phase promoting factor (MPF) and the M phase-specific Histone H1 kinase. The p34Cdc2/cyclin B complex is required for the G2 to M transition. An additional cell cycle-dependent protein kinase, termed p55cdc, exhibits a high degree of homology with the S. cerevisiae proteins Cdc20 and Cdc4. The p55cdc transcript is readily detectable in a variety of cultured cell lines in growth phase, but disappears when cell growth is chemically arrested.
Alternate name: Fizzy protein, CDC2, CDC2A, p55CDC, P55CDC-LSBHuman Entrez Gene ID 991 Human SwissProt Q12834 Human Unigene 524947 Human Gene Symbol CDC2Human Chromosome Location 1p34.2
Conjugation: Unconjugated
Isotype: IgG1 kappa
Molecular weight: 55 kDa
Immunogen: Urea-denatured His6 human Cdc20 recombinant protein
Myeloma used: P3X63Ag8.653
Cat. #: 151310
Tool sub type: Primary antibody
Unit size: 100 ug
Research Fields: Biochemistry;Cell biology
Application: FACS ; IHC ; IF ; IP
Target: Cell division cycle protein 20 (cdc20)
Reactivity: Human
Clone: AR12.4
Host: Mouse
Class: Monoclonal
Target background: Cdc20 (fizzy protein) exhibits protein kinase activity in vitro and exists in a complex with both cyclin B and a protein homologous to p13SUC1. Cdc2 kinase is the active subunit of the M phase promoting factor (MPF) and the M phase-specific Histone H1 kinase. The p34Cdc2/cyclin B complex is required for the G2 to M transition. An additional cell cycle-dependent protein kinase, termed p55cdc, exhibits a high degree of homology with the S. cerevisiae proteins Cdc20 and Cdc4. The p55cdc transcript is readily detectable in a variety of cultured cell lines in growth phase, but disappears when cell growth is chemically arrested.
Format: Liquid
Concentration: 1 mg/ml
Storage buffer: PBS with 0.02% azide
Storage conditions: -15° C to -25° C
Shipping conditions: Dry ice
References:

Sironi et al. 2001. EMBO J. 20(22):6371-82. PMID: 11707408.

Mad2 binding to Mad1 and Cdc20, rather than oligomerization, is required for the spindle checkpoint.

Images

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Formalin-fixed; paraffin-embedded human Gastric Carcinoma stained with CDC20 Monoclonal Antibody (AR12)

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