GST-tagged human cannabinoid CB1 receptor (amino acids 414 to 442)

Purified GST-CB1414–442 fusion protein

Contributors

Inventor Institute
Gontzal García del Caño University of the Basque Country
Cat. #: 161589
Tool sub type: GST fusion protein
Research Fields: Biochemistry;Cell biology;Molecular Biology;Neurobiology
Application: Negative control for quantitative Western blot to determine CB1 receptor density in biological samples using antibodies raised against peptides within the sequence spanning amino acids 443-472 at the C-terminus of the CB1 receptor. Negative control in protein-protein interaction assays (e.g., GST pull-down surface plasmon resonance) involving proteins interacting with the CB1 receptor at the sequence spanning amino acids 414-472 of the C-terminal end of the CB1 receptor.
Primary citation: Saumell-Esnaola M et al. 2022. Microb Cell Fact. 21(1):192. PMID: 36109736.
Product description: Polypeptide spanning amino acids 414 to 442 (CB1414–442) of the cytosolic tail of the human cannaboinoid CB1 receptor fused to the C-terminus of the glutathione S-transferase (GST) tag. The linker between the GST tag and the CB1414–442 polyopeptide posses the consensus cleavage site LeuPheGlnGlyPro for the PreScission protease for removing the GST tag after purification.
Additional notes: Contact the inventor for further information.
Molecular weight: 29710.86 Da
Tag: Glutathione S-transferase (GST)
Expression system: Escherichia coli
Format: Solution
Storage buffer: 126 mM Tris-HCl, 150 mM NaCl, 1 mM DTT, 1 mM EDTA, pH 7.4Contains 50 mM reduced glutathione which may need to be removed (e.g. by dialysis) for certain assays.
Storage conditions: -80° C
References:

Saumell-Esnaola et al. 2022 Microb Cell Fact.21(1):192. PMID: 36109736

Gómez-Caballero et al. 2021. Mikrochim Acta. 188(11):368. PMID: 34618242.

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