#161589

GST-tagged human cannabinoid CB1 receptor (amino acids 414 to 442)

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Cat. #161589

GST-tagged human cannabinoid CB1 receptor (amino acids 414 to 442)

Cat. #: 161589

Sub-type: GST fusion protein

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Contributor

Inventor: Gontzal García del Caño

Institute: University of the Basque Country

Primary Citation: Saumell-Esnaola M et al. 2022. Microb Cell Fact. 21(1):192. PMID: 36109736.

Tool Details
Handling
Application Details
References

Tool Details

*FOR RESEARCH USE ONLY

  • Tool name: GST-tagged human cannabinoid CB1 receptor (amino acids 414 to 442)
  • Research fields: Biochemistry;Cell biology;Molecular Biology;Neurobiology
  • Tool sub type: GST fusion protein
  • Sequence: Saumell-Esnaola M et al. 2022. Microb Cell Fact. 21(1):192. PMID: 36109736.
  • Description: Polypeptide spanning amino acids 414 to 442 (CB1414–442) of the cytosolic tail of the human cannaboinoid CB1 receptor fused to the C-terminus of the glutathione S-transferase (GST) tag. The linker between the GST tag and the CB1414–442 polyopeptide posses the consensus cleavage site LeuPheGlnGlyPro for the PreScission protease for removing the GST tag after purification.
  • Application: Negative control for quantitative Western blot to determine CB1 receptor density in biological samples using antibodies raised against peptides within the sequence spanning amino acids 443-472 at the C-terminus of the CB1 receptor. Negative control in protein-protein interaction assays (e.g., GST pull-down surface plasmon resonance) involving proteins interacting with the CB1 receptor at the sequence spanning amino acids 414-472 of the C-terminal end of the CB1 receptor.
  • Tag: Glutathione S-transferase (GST)
  • Molecular weight: 29710.86 Da
  • Expression system: Escherichia coli
  • Additional notes: Contact the inventor for further information.

Handling

  • Format: Solution
  • Purity: Affinity purified
  • Storage conditions: -80° C
  • Storage buffer: 126 mM Tris-HCl, 150 mM NaCl, 1 mM DTT, 1 mM EDTA, pH 7.4Contains 50 mM reduced glutathione which may need to be removed (e.g. by dialysis) for certain assays.
  • Shipping conditions: Frozen in dry ice

Application Details

  • Application: Negative control for quantitative Western blot to determine CB1 receptor density in biological samples using antibodies raised against peptides within the sequence spanning amino acids 443-472 at the C-terminus of the CB1 receptor. Negative control in protein-protein interaction assays (e.g., GST pull-down surface plasmon resonance) involving proteins interacting with the CB1 receptor at the sequence spanning amino acids 414-472 of the C-terminal end of the CB1 receptor.
  • Application notes: Negative control for quantitative Western blot to determine CB1 receptor density in biological samples using antibodies raised against peptides within the sequence spanning amino acids 443-472 at the C-terminus of the CB1 receptor. Negative control in protein-protein interaction assays (e.g., GST pull-down surface plasmon resonance) involving proteins interacting with the CB1 receptor at the sequence spanning amino acids 414-472 of the C-terminal end of the CB1 receptor.

References

  • Saumell-Esnaola et al. 2022 Microb Cell Fact.21(1):192. PMID: 36109736
  • Gómez-Caballero et al. 2021. Mikrochim Acta. 188(11):368. PMID: 34618242.