Cat. #161589
GST-tagged human cannabinoid CB1 receptor (amino acids 414 to 442)
Cat. #: 161589
Sub-type: GST fusion protein
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Gontzal García del Caño
Institute: University of the Basque Country
Primary Citation: Saumell-Esnaola M et al. 2022. Microb Cell Fact. 21(1):192. PMID: 36109736.
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Tool name: GST-tagged human cannabinoid CB1 receptor (amino acids 414 to 442)
- Research fields: Biochemistry;Cell biology;Molecular Biology;Neurobiology
- Tool sub type: GST fusion protein
- Sequence: Saumell-Esnaola M et al. 2022. Microb Cell Fact. 21(1):192. PMID: 36109736.
- Description: Polypeptide spanning amino acids 414 to 442 (CB1414–442) of the cytosolic tail of the human cannaboinoid CB1 receptor fused to the C-terminus of the glutathione S-transferase (GST) tag. The linker between the GST tag and the CB1414–442 polyopeptide posses the consensus cleavage site LeuPheGlnGlyPro for the PreScission protease for removing the GST tag after purification.
- Application: Negative control for quantitative Western blot to determine CB1 receptor density in biological samples using antibodies raised against peptides within the sequence spanning amino acids 443-472 at the C-terminus of the CB1 receptor. Negative control in protein-protein interaction assays (e.g., GST pull-down surface plasmon resonance) involving proteins interacting with the CB1 receptor at the sequence spanning amino acids 414-472 of the C-terminal end of the CB1 receptor.
- Tag: Glutathione S-transferase (GST)
- Molecular weight: 29710.86 Da
- Expression system: Escherichia coli
- Additional notes: Contact the inventor for further information.
Handling
- Format: Solution
- Purity: Affinity purified
- Storage conditions: -80° C
- Storage buffer: 126 mM Tris-HCl, 150 mM NaCl, 1 mM DTT, 1 mM EDTA, pH 7.4Contains 50 mM reduced glutathione which may need to be removed (e.g. by dialysis) for certain assays.
- Shipping conditions: Frozen in dry ice
Application Details
- Application: Negative control for quantitative Western blot to determine CB1 receptor density in biological samples using antibodies raised against peptides within the sequence spanning amino acids 443-472 at the C-terminus of the CB1 receptor. Negative control in protein-protein interaction assays (e.g., GST pull-down surface plasmon resonance) involving proteins interacting with the CB1 receptor at the sequence spanning amino acids 414-472 of the C-terminal end of the CB1 receptor.
- Application notes: Negative control for quantitative Western blot to determine CB1 receptor density in biological samples using antibodies raised against peptides within the sequence spanning amino acids 443-472 at the C-terminus of the CB1 receptor. Negative control in protein-protein interaction assays (e.g., GST pull-down surface plasmon resonance) involving proteins interacting with the CB1 receptor at the sequence spanning amino acids 414-472 of the C-terminal end of the CB1 receptor.
References
- Saumell-Esnaola et al. 2022 Microb Cell Fact.21(1):192. PMID: 36109736
- Gómez-Caballero et al. 2021. Mikrochim Acta. 188(11):368. PMID: 34618242.