#151773

HBRN Mouse

Cat. #151773

HBRN Mouse

Cat. #: 151773

Sub-type: Mouse

Availability: 6-8 weeks

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Inventor: Colin Henderson ; Roland Wolf

Institute: University of Dundee

Tool Details
Handling
Target Details
References

Tool Details

*FOR RESEARCH USE ONLY

  • Tool name: HBRN Mouse
  • Research fields: Cell signaling and signal transduction;Genetics;Metabolism
  • Tool sub type: Mouse
  • Conditional: No
  • Description: Improved model of abrogation of hepatic P450 function. The dual deletion of both Cyb5 and POR almost completely abrogates all hepatic cytochrome P450 activities; the new HBRN model thus provides a more authentic hepatic-P450 null phenotype
  • Genetic background: HBRN (Cytb5lox/lox::PORlox/lox CreALB) and wild-type (WT; PORlox/lox::Cyb5lox/lox) were generated by crossing HRN (PORlox/lox + CreALB ) and floxed cytochrome b5 mice (Cytb5lox/lox), and thereafter maintained by crossing of homozygous pairs within each line.
  • Zygosity: HBRN (Cytb5lox/lox::PORlox/lox ą CreALB)
  • Production details: HBRN (Cytb5lox/lox::PORlox/lox Âą CreALB) and wild-type (WT; PORlox/lox::Cyb5lox/lox) were generated by crossing HRN (PORlox/lox + CreALB ) and floxed cytochrome b5 mice (Cytb5lox/lox), and thereafter maintained by crossing of homozygous pairs within each line.

Handling

  • Shipping conditions: Embryo/Spermatoza- Dry Ice

Target Details

  • Target: P450, Cyb5

References

  • Henderson et al. 2013. Mol Pharmacol. 83(6):1209-17. PMID: 23530090.
  • Evidence that cytochrome b5 and cytochrome b5 reductase can act as sole electron donors to the hepatic cytochrome P450 system.
  • Henderson et al. 2003. J Biol Chem. 278(15):13480-6. PMID: 12566435.
  • Inactivation of the hepatic cytochrome P450 system by conditional deletion of hepatic cytochrome P450 reductase.