ZR-75-1 PDGFRB [ZR-PDGFRB (4147)] cell line

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

• Name: ZR-75-1 PDGFRB [ZR-PDGFRB (4147)] cell line
• Alternate name: Platelet Derived Growth Factor Receptor Beta; PDGFR1; CD14B
• Cancer: Gynaecologic cancer
• Cancers detailed: Mammary ductal carcinoma
• Research fields: Cancer;Drug development
• Tool sub type: Continuous
• Parental cell: ZR-75-1
• Organism: Human
• Tissue: Breast
• Disease: Cancer
• Model: Cancer Model
• Description: Breast cancer is widely and effectively treated with endocrine treatment. However, in advanced disease cases the tumours will eventually progress into an estrogen-independent and therapy-resistant phenotype.Seven genes including AKT1, AKT2, BCAR1, BCAR2, BCAR3, EGFR2 and GRB7 have been shown to directly underlie estrogen independence in human breast cancer cells. This cell line is part of a panel of 16 cell lines (Cat No 154621-154635, 154642) which have been transfected with these genes, plus the parental (Cat No 154547).
• Production details: Retroviral particles containing PDGFRB cDNA were produced by transient transfection of Phoenix-Ampho packaging cells using FuGENE6. ZR-75-1 cells were incubated with viral particles in the presence of 4ug/ml polybrene and 1nmol/L estradiol. Three days after transfection cells were cultured in medium supplemented with G418 and resistant colonies were expanded.
• Biosafety level: 1

Target Details

• Target: PDGFRB

Handling

• Format: Frozen
• Growth medium: RPMI 1640 medium supplemented with 10% heat-inactivated bovine serum (RBCS) and 1 nM 17?-estradiol
• Unit size: 1x10^6 cells / vial
• Shipping conditions: Dry ice
• Storage conditions: Liquid Nitrogen
• Mycoplasma free: Yes

References

• van Agthoven et al. 2010. Endocr Relat Cancer. 17(1):215-30. PMID: 19966015.