U2OS PBRM1 KO2

HEK293TN cell line knockout for ARID2 knockout (clone 21, also called A21)

Contributors

Inventor Institute
Jessica Downs, Hugang Freng, Karen Lane The Institute of Cancer Research
Cat. #: 161883
Cancer types: Sarcoma
Research Fields: Cancer
Organism: Human
Tissue: bone
Gender: Female
Model: Genetically modified
Morphology: Epithelial-like
Growth properties: Adherent
Cancer Types In Detail: Osteosarcoma
Product description: U2OS cell line knockout for PBRM1 (clone 2). This cell line was generated using CRISPR-Cas9 engineering to create a loss of function mutation in the indicated subunit of the SWI/SNF chromatin remodelling complex. The cell line was validated using Sanger sequencing, immunofluorescence, and proteomic analysis.
Gender: Female
CRISPR: Yes
Conditional: No
Cellosaurus ID: CVCL_0042
Parental cell line: U2OS
Model description: PBRM1 knockout
Products or characteristics of interest: Gene loss-of-function, chromatin-remodelling, DNA damage, cell cycle response, regulation of t-cell-mediated cell killing.
Format: Frozen
Storage conditions: Liquid Nitrogen
Shipping conditions: Dry ice
Growth medium: McCoys 5A =10S + 1% Penicillin/Streptomycin
Temperature: 37C
Atmosphere: 5% CO2 in air
Biosafety level: 1
References:

Unpublished

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