MEF ULK2 KO (SV40) Cell Line

MEF ULK2 KO (SV40) cell line provides a tool for the study of Ulk2 and of Autophagy and has a comparable genetic background to MEF Ulk1 Ulk2 DKO and MEF

Contributors

Inventor Institute
Sharon Tooze Cancer Research UK, London Research Institute: Lincoln's Inn Fields
SKU: 152610
Product description: The MEF ULK2 KO (SV40) cell line provides a tool for the study of Ulk2 and of Autophagy and has a comparable genetic background to MEF Ulk1 Ulk2 DKO and MEF Ulk1 KO cell lines. This cell line is derived from an embryo with a mixed genetic background (Blk6/129B Agouti) and immortalized with SV40 large T-antigen.
Production details: Primary embryonic fibroblasts were isolated from the embryos of a pregnant female mouse at day 13p.c. The embryos were genotyped to identify those that were Ulk1 +/+ Ulk2 -/- and the MEFs that were isolated and cultured were immortalised SV40 using a standard serial passaging protocol.
Cellosaurus ID: CVCL_5A55
Disease: Cancer
Cat. #: 152610
Provenance and Ownership: The MEF ULK2 KO (SV40) Cell Line is owned by Cancer Research UK. CancerTools supplies the MEF ULK2 KO (SV40) Cell Line under licence from Cancer Research UK and is the authorised source for its distribution. Sourcing MEF ULK2 KO (SV40) Cell Line directly from the IP owner ensures authenticity, provenance and compliance with all applicable licensing and research use requirements.
Unit size: 1×10^6 cells / vial
Research Fields: Apoptosis and autophagy; Cancer; Cell signaling and signal transduction; Metabolism; Neurobiology
Organism: Mouse
Tissue: Embryo
Model: Knock-Out
Growth properties: Fibroblast
Format: Frozen
Shipping conditions: Dry ice
Growth medium: DMEM + 20% FCS + 2mM Glutamine + pen/strep
References:

McAlpine et al. 2013. Autophagy. 9(3):361-73. PMID: 23291478.

Regulation of nutrient-sensitive autophagy by uncoordinated 51-like kinases 1 and 2.

Images

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MEF ULK2 KO (SV40) Cell Line. 3 days post plating. Image courtesy of the European Collection of Authenticated Cell Cultures (ECACC)

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