MEF ULK1 KO (SV40) Cell Line

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This cell line is derived from an embryo with a mixed genetic background (Blk6/129 Agouti) and has been immortalised with SV40 large T-antigen.

Contributors

Inventor Institute
Sharon Tooze Cancer Research UK, London Research Institute: Lincoln's Inn Fields
SKU: 152609
Product description: The MEF ULK1 KO (SV40) cell line provides a tool for the study of Ulk1 and of Autophagy and has a comparable genetic background to MEF Ulk1 Ulk2 DKO and MEF Ulk2 KO cell lines. This cell line is derived from an embryo with a mixed genetic background (Blk6/129 Agouti) and has been immortalised with SV40 large T-antigen.
Conditional: Yes
Production details: Primary embryonic fibroblasts were isolated from the embryos of a pregnant female Ulk1-/- Ulk2-/+ mouse at day 13p.c. The embryos were genotyped to identify those that were Ulk1 -/- Ulk2 +/+ and the MEFs that were isolated and cultured were immortalised by SV40 using a standard serial passaging protocol.
Cellosaurus ID: CVCL_5A53
Disease: Cancer
Cat. #: 152609
Provenance and Ownership: The MEF ULK1 KO (SV40) Cell Line is owned by Cancer Research UK. CancerTools supplies the MEF ULK1 KO (SV40) Cell Line under licence from Cancer Research UK and is the authorised source for its distribution. Sourcing MEF ULK1 KO (SV40) Cell Line directly from the IP owner ensures authenticity, provenance and compliance with all applicable licensing and research use requirements.
Unit size: 1×10^6 cells / vial
Research Fields: Apoptosis and autophagy;Cancer;Cell signaling and signal transduction;Metabolism;Neurobiology
Organism: Mouse
Tissue: Embryo
Model: Knock-Out
Growth properties: Fibroblast
Format: Frozen
Shipping conditions: Dry ice
Growth medium: DMEM + 20% FCS + 2mM Glutamine + pen/strep
References:

McAlpine et al. 2013. Autophagy. 9(3):361-73. PMID: 23291478.

Regulation of nutrient-sensitive autophagy by uncoordinated 51-like kinases 1 and 2.

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Related Tools for Cell lines

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151715 MEF ULK1 KO (SIM) Cell Line Key Info View Tool
151776 MEF ULK2 KO (SIM) Cell Line Key Info View Tool
151714 MEF ULK1/2 WT (SIM) Cell Line Key Info View Tool
151774 MEF ULK1 ULK2 DKO (SIM) Cell Line Key Info View Tool
152607 MEF ULK1/2 WT(SV40) Cell Line Key Info View Tool
152608 MEF ULK1 ULK2 DKO (SV40) Cell Line Key Info View Tool
152610 MEF ULK2 KO (SV40) Cell Line Key Info View Tool

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