MEF ULK1 ULK2 DKO (SV40) Cell Line

The MEF ULK1 ULK2 DKO (SV40) cell line provides a tool for the study of Ulk1 and Ulk2 and of Autophagy.

Contributors

Inventor Institute
Sharon Tooze Cancer Research UK, London Research Institute: Lincoln's Inn Fields
Cat. #: 152608
Unit size: 1×10^6 cells / vial
Research Fields: Apoptosis and autophagy;Cancer;Cell signaling and signal transduction;Metabolism;Neurobiology
Organism: Mouse
Tissue: Embryo
Model: Knock-Out
Growth properties: Fibroblast
Alternate name: MEF Ulk1 -/- Ulk2 -/- (DKO) (SV40)
Product description: MEF ULK1 ULK2 DKO (SV40) cells were immortalised with SV40 large T-antigen. This mouse embryonic fibroblast Ulk1/Ulk2 double knock-out cell line is from an embryo with a mixed genetic background (Blk6/129 Agouti). The MEF ULK1 ULK2 DKO (SIM) cell line provides a tool for the study of Ulk1 and Ulk2 and of autophagy. Autophagy has been implicated in cancer, neurodegeneration and immune-related disorders. Discover our group of mouse embryonic fibroblast cell lines for autophagy research.
Conditional: Yes
Production details: Primary embryonic fibroblasts were isolated from the embryos of a pregnant female Ulk1-/- Ulk2-/+ mouse at day 13 p.c. The embryos were genotyped to identify those that were Ulk1 -/- Ulk2 -/- and the MEFs that were isolated and cultured were immortalised by SV40 using a standard serial passaging protocol.
Cellosaurus ID: CVCL_5A57
disease: Cancer
Format: Frozen
Shipping conditions: Dry ice
Growth medium: DMEM + 10% FCS + 2mM Glutamine
Subculture routine: Trypsinise with 0.05% Trypsin/EDTA and inoculate new cultures at 2 x 10^4 per cm². Cultures must be incubated in a humidified 5% CO₂/95% air incubator at 37°C.
References:

McAlpine et al. 2013. Autophagy. 9(3):361-73. PMID: 23291478.

Regulation of nutrient-sensitive autophagy by uncoordinated 51-like kinases 1 and 2.

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