MEF ULK1 ULK2 DKO (SV40) Cell Line
The MEF ULK1 ULK2 DKO (SV40) cell line provides a tool for the study of Ulk1 and Ulk2 and of Autophagy.
Contributors
| Inventor | Institute |
|---|---|
| Sharon Tooze | Cancer Research UK, London Research Institute: Lincoln's Inn Fields |
| Cat. #: | 152608 |
|---|---|
| Unit size: | 1×10^6 cells / vial |
| Research Fields: | Apoptosis and autophagy;Cancer;Cell signaling and signal transduction;Metabolism;Neurobiology |
| Organism: | Mouse |
| Tissue: | Embryo |
| Model: | Knock-Out |
| Growth properties: | Fibroblast |
| Alternate name: | MEF Ulk1 -/- Ulk2 -/- (DKO) (SV40) |
|---|---|
| Product description: | MEF ULK1 ULK2 DKO (SV40) cells were immortalised with SV40 large T-antigen. This mouse embryonic fibroblast Ulk1/Ulk2 double knock-out cell line is from an embryo with a mixed genetic background (Blk6/129 Agouti). The MEF ULK1 ULK2 DKO (SIM) cell line provides a tool for the study of Ulk1 and Ulk2 and of autophagy. Autophagy has been implicated in cancer, neurodegeneration and immune-related disorders. Discover our group of mouse embryonic fibroblast cell lines for autophagy research. |
| Conditional: | Yes |
| Production details: | Primary embryonic fibroblasts were isolated from the embryos of a pregnant female Ulk1-/- Ulk2-/+ mouse at day 13 p.c. The embryos were genotyped to identify those that were Ulk1 -/- Ulk2 -/- and the MEFs that were isolated and cultured were immortalised by SV40 using a standard serial passaging protocol. |
| Cellosaurus ID: | CVCL_5A57 |
| disease: | Cancer |
| Format: | Frozen |
|---|---|
| Shipping conditions: | Dry ice |
| Growth medium: | DMEM + 10% FCS + 2mM Glutamine |
| Subculture routine: | Trypsinise with 0.05% Trypsin/EDTA and inoculate new cultures at 2 x 10^4 per cm². Cultures must be incubated in a humidified 5% CO₂/95% air incubator at 37°C. |
| References: |
McAlpine et al. 2013. Autophagy. 9(3):361-73. PMID: 23291478. Regulation of nutrient-sensitive autophagy by uncoordinated 51-like kinases 1 and 2. |
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