Cat. #152608
MEF ULK1 ULK2 DKO (SV40) Cell Line
Cat. #: 152608
Unit size: 1x10^6 cells / vial
Availability: 10-12 weeks
Organism: Mouse
Tissue: Embryo
Disease: Cancer
Model: Knock-Out
£575.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Sharon Tooze
Institute: Cancer Research UK, London Research Institute: Lincoln's Inn Fields
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: MEF ULK1 ULK2 DKO (SV40) Cell Line
- Alternate name: MEF Ulk1 -/- Ulk2 -/- (DKO) (SV40)
- Research fields: Apoptosis and autophagy;Cancer;Cell signaling and signal transduction;Metabolism;Neurobiology
- Organism: Mouse
- Tissue: Embryo
- Disease: Cancer
- Growth properties: Fibroblast
- Model: Knock-Out
- Conditional: Yes
- Description: MEF ULK1 ULK2 DKO (SV40) cells were immortalised with SV40 large T-antigen. This mouse embryonic fibroblast Ulk1/Ulk2 double knock-out cell line is from an embryo with a mixed genetic background (Blk6/129 Agouti). The MEF ULK1 ULK2 DKO (SIM) cell line provides a tool for the study of Ulk1 and Ulk2 and of autophagy. Autophagy has been implicated in cancer, neurodegeneration and immune-related disorders. Discover our group of mouse embryonic fibroblast cell lines for autophagy research.
- Production details: Primary embryonic fibroblasts were isolated from the embryos of a pregnant female Ulk1-/- Ulk2-/+ mouse at day 13 p.c. The embryos were genotyped to identify those that were Ulk1 -/- Ulk2 -/- and the MEFs that were isolated and cultured were immortalised by SV40 using a standard serial passaging protocol.
- Cellosaurus id: CVCL_5A57
Target Details
- Target: ULK1, ULK2
Handling
- Format: Frozen
- Growth medium: DMEM + 10% FCS + 2mM Glutamine
- Unit size: 1x10^6 cells / vial
- Shipping conditions: Dry ice
- Subculture routine: Trypsinise with 0.05% Trypsin/EDTA and inoculate new cultures at 2 x 10^4 per cm². Cultures must be incubated in a humidified 5% CO₂/95% air incubator at 37°C.
Related Tools
- Related tools: MEF ULK1/2 WT (SIM) Cell Line ; MEF ULK1 KO (SIM) Cell Line ; MEF ULK1/2 WT(SV40) Cell Line ; MEF ULK2 KO (SIM) Cell Line ; MEF ULK1 ULK2 DKO (SIM) Cell Line ; MEF ULK1 KO (SV40) Cell Line ; MEF ULK2 KO (SV40) Cell Line
References
- McAlpine et al. 2013. Autophagy. 9(3):361-73. PMID: 23291478.
- Regulation of nutrient-sensitive autophagy by uncoordinated 51-like kinases 1 and 2.
