MCF7/S0.5 Cell Line
The MCF7/S0.5 cell line has been established to grow at low serum. the MCF-7/S0.5 cell line has been adapted to long-term growth at 0.5 % fetal bovine serum in order to reduce the oestrogen supply via serum and to enable oestrogen receptor-mediated growth inhibition with the antiestrogen tamoxifen._x000D_ _x000D_ The MCF7/S0.5 cells are also useful for studies of effect of oestrogens, if they, instead of 1 % fetal calf serum, are cultivated with 10% newborn calf serum which contains very low oestrogenic activity.
Contributors
| Inventor | Institute |
|---|---|
| Anne Lykkesfeldt | Danish Cancer Society |
| Cat. #: | 152090 |
|---|---|
| Tool sub type: | Continuous |
| Unit size: | 1×10^6 cells / vial |
| Cancer types: | Breast cancer |
| Research Fields: | Cancer;Drug development |
| Organism: | Human |
| Tissue: | Breast |
| Gender: | Female |
| Model: | Tumour line |
| Morphology: | Epithelial; Adherent polygonal epithelia |
| Growth properties: | Adherent |
| Cancer Types In Detail: | Breast cancer |
| Primary citation: | Briand et al. 1984. Cancer Res. 44(3):1114-9. PMID: 6362856 |
| Alternate name: | MCF7/S0.5; MCF-7/S0.5; MCF7 S0.5 |
|---|---|
| Product description: | The MCF7/S0.5 human breast cancer cell line has been established to grow at low serum. The presence of hormones in the serum required in tissue culture media complicates the demonstration of specific hormone and antihormone effects. Methods to overcome this have included charcoal-treated serum and serum-free medium. However, both methods have serious drawbacks. Therefore, the MCF-7/S0.5 cell line has been adapted to long-term growth at 0.5 % fetal bovine serum in order to reduce the oestrogen supply via serum and to enable oestrogen receptor-mediated growth inhibition with the anti-oestrogens like tamoxifen. The MCF7/S0.5 cells are also useful for studies investigating the effects of oestrogens, if they, instead of 1% fetal calf serum, are cultivated with 10% newborn calf serum which contains very low oestrogenic activity. MCF7/S0.5 cells express both oestrogen and progesterone receptors. Many tamoxifen, fulvestrant, letrozole, anastrozole, and exemestane resistant cell lines have been derived from MCF7/S0.5 and so this line can be utilized as a parental sensitive control to its derivative resistant cell lines in parallel experiments. |
| Gender: | Female |
| CRISPR: | No |
| Conditional: | Yes |
| Production details: | This MCF7 subline was established buy stepwise reduction in the serum concentration from 5% fetal calf serum to 0.5 % in growth medium consisting of DMEM: Ham's F-12 medium (1:1) supplemented with 2mM glutamine and insulin (6ng/ml) |
| Cellosaurus ID: | CVCL_1D47 |
| Receptors of note: | Oestrogen receptor and progesterone receptors |
| Parental cell line: | MCF7 |
| disease: | Cancer |
| Format: | Frozen |
|---|---|
| Storage conditions: | Liquid Nitrogen |
| Shipping conditions: | Dry ice |
| Growth medium: | Phenol-red-free DMEM/F12 medium supplemented with 1% Fetal calf serum 2.5 mM Glutamax and 6 ng/ ml Insulin |
| Subculture routine: | Split at 70-80% confluence: 1:3 to 1:6 (seeding at 2-4×104 cells/cm2) using 0.05% trypsin or trypsin or 0.05% trypsin/EDTA solution. Change the media every 2-3 days |
| Temperature: | 37° C |
| Atmosphere: | 5% CO2 |
| Mycoplasma free: | Yes |
| Biosafety level: | 1 |
| References: |
Joshi et al. 2016. Oncotarget. :. PMID: 27528030 Alves et al. 2016. Clin Cancer Res. :. PMID: 27252418 Elias et al. 2015. Oncogene. 34:1919–1927. PMID: 24882577 Thomsen et al. 2015. Breast Cancer Res Treat. :. PMID: 26585578 Hole et al. 2015. Int J Oncol. 46(4):1481-90. PMID: 25625755 Sonne-Hansen et al. 2005. J Steroid Biochem Mol Biol. 93(1):25-34. PMID: 15748829 Lykkesfeldt et al. 1995. Int J Cancer. 61(4):529-34. PMID: 7759159 Madsen et. al. 1995. Mol Cell Endocrinol 109(2):197-207. PMID: 7664983 Lykkesfeldt et al. 1994. Cancer Res. 54(6):1587-95. PMID: 8137264 Lykkesfeldt et al. 1989. Mol Cell Endocrinol. 62(2):287-96. PMID: 2744230 Lykkesfeldt et al. 1986. Br J Cancer. 53(1):29-35. PMID: 3947513 Briand et al. 1984. Cancer Res. 44(3):1114-9. PMID: 6362856. |
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