Cells grow as adherent monolayer cultures under standard culture conditions at 37°C in a humidified incubator with 5% CO2. Medium is replaced twice per week.
Primary citation:
Campos et al. 2015. Glia. 63(10):1850-1859. PMID: 25944104.
Product description:
Glioblastoma WHO IV cell line, NCH676
Production details:
Patient-derived cell line established from human tumour tissue obtained during surgical resection and expanded in vitro.
Growth medium:
DMEM (high glucose) supplemented with 10% fetal calf serum (FCS), 1% GlutaMAX, and 1% penicillin/streptomycin.
Subculture routine:
Cells are passaged at sub-confluent densities using 0.05% Trypsin-EDTA. After enzymatic detachment, trypsin is neutralised with serum-containing medium and cells are reseeded into fresh culture vessels. Passage number should be recorded.
Temperature:
37°C
References:
Campos et al. 2015. Glia. 63(10):1850-1859. PMID: 25944104.
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