Caki-2 DNMT3A KO cell line

Experimental ablation of DNMT3A gene expression using the CRISPR/Cas9 system renders Caki-2-DNMT3A knockout cells a valuable tool to investigate the role of DNA methylation in processes such as cell differentiation or cancer progression.

Contributors

Inventor Institute
Sergio F de Almeida Instituto de Medicina Molecular Jo?„ƒo Lobo Antunes
Cat. #: 160723
Tool sub type: Continuous
Unit size: 1×10^6 cells / vial
Research Fields: Cancer;Genetics
Organism: Human
Tissue: Kidney
Model: Knock-Out
Alternate name: TBRS, HESJAS
Product description: Experimental ablation of DNMT3A gene expression using the CRISPR/Cas9 system renders Caki-2-DNMT3A knockout cells a valuable tool to investigate the role of DNA methylation in processes such as cell differentiation or cancer progression.
Conditional: No
Production details: CRISPR/Cas9 KO
Additional notes: Cancer Research Technology Limited (trading research tools as Ximbio) has been granted a non-exclusive license to the CRISPR-Cas9 technology by ERS Genomics Ltd under the patent rights listed here. This license from ERS Genomics Ltd allows Ximbio to develop and commercialise CRISPR-Cas9 modified cell lines for research use only. Ximbio can provide these modified CRISPR-Ca…
Parental cell line: Clear cell renal cell carcinoma cell line (Caki-2)
Format: Frozen
Shipping conditions: Dry ice
Growth medium: Grow as monolayers in Dulbecco's modified Eagle medium, supplemented with 10% (v/v) FBS, 1% (v/v) nonessential amino acids, 1% (v/v) L-glutamine and 100U/mL penicillin-streptomycin, at 37?‚°C in a humidified atmosphere with 5% CO2.
Recommended controls: Caki-2 Parental Line
References:

de Matos et al. 2019. Cancers (Basel). 11(3):. PMID: 30897760.

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