Caki-2 SETD2 KO cell line

Experimental ablation of SETD2 gene expression using the CRISPR/Cas9 system renders Caki-2-SETD2 knockout cells a valuable tool to investigate the role of H3K36me3 histone methylation in processes such as transcription or DNA repair.

Contributors

Inventor Institute
Sergio F de Almeida Instituto de Medicina Molecular Jo?„ƒo Lobo Antunes
SKU: 160724
Product description: Experimental ablation of SETD2 gene expression using the CRISPR/Cas9 system renders Caki-2-SETD2 knockout cells a valuable tool to investigate the role of H3K36me3 histone methylation in processes such as transcription or DNA repair.
Alternate name: HBP231, HIF-1, HIP-1, HYPB, KMT3A
Conditional: No
Production details: CRISPR/Cas9 KO
Additional notes: Cancer Research Technology Limited (trading research tools as Ximbio) has been granted a non-exclusive license to the CRISPR-Cas9 technology by ERS Genomics Ltd under the patent rights listed here. This license from ERS Genomics Ltd allows Ximbio to develop and commercialise CRISPR-Cas9 modified cell lines for research use only. Ximbio can provide these modified CRISPR-Ca…
Parental cell line: Clear cell renal cell carcinoma cell line (Caki-2)
Cat. #: 160724
Tool sub type: Continuous
Unit size: 1×10^6 cells / vial
Cancer types: Kidney cancer
Research Fields: Cancer; Genetics
Organism: Human
Tissue: Kidney
Model: Knock-Out
Format: Frozen
Shipping conditions: Dry ice
Growth medium: Grow as monolayers in Dulbecco’s modified Eagle medium, supplemented with 10% (v/v) FBS, 1% (v/v) nonessential amino acids, 1% (v/v) L-glutamine and 100U/mL penicillin-streptomycin, at 37?‚°C in a humidified atmosphere with 5% CO2.
Recommended controls: Caki-2 Parental Line
References:

de Matos et al. 2019. Cancers (Basel). 11(3):. PMID: 30897760.

Related Tools for Cell lines

Cat. # Tool Name
151014 MCF7 AREc32 Cell Line Key Info View Tool
151463 Omega-E Packaging Cell Line Key Info View Tool
151479 HCT 116 p300 KO [D10] Cell Line Key Info View Tool
151480 HEK293A EGFP-LC3 Cell Line Key Info View Tool
151503 1-7 CE1 Cell Line Key Info View Tool

Related News

UM-UC-10 Cell Line. Image courtesy of the European Collection of Authenticated Cell Cultures (ECACC), UK.
Cell Lines

Popular cell lines now available only from CancerTools.org

Read More
Cell Lines

New cell lines: murine melanocyte and melanoblast cell lines from Dr. Elena Sviderskaya

Read More
Image Reference: 2x PTEN -/- mouse prostate cancer cell lines (MuCap system). Histology staining of syngraft tumours (Van Duijn et al., 2018).
Cell Lines

38 new prostate cancer cell lines

Read More

Tool enquiry

Please ensure you use your organisation email address rather than personal where possible, as this helps us locate your organisation in our system faster.

Please note we may take up to three days to respond to your enquiry.

CancerTools.org uses the contact information provided to respond to you about our research tools and service. For more information please review our privacy policy.