C12 Cell Line Overexpressing Human P2X3
Cell Line is a clone that expresses hP2X3 in a HEK293 cell background
Contributors
| Inventor | Institute |
|---|---|
| Philippe SeguelaRuben Ariel R Ase | McGill University |
| Cat. #: | 162167 |
|---|---|
| Research Fields: | Cell biology |
| Organism: | Human |
| Tissue: | Kidney |
| Gender: | Female |
| Model: | Genetically modified cell line |
| Morphology: | Epithelial morphology, typically less circular than other common cell lines, and they often display dendritic-like processes. They are slightly granular, and they tend to be adherent. |
| Growth properties: | Adherent |
| Product description: | Cell Line is a clone that expresses hP2X3 in a HEK293 cell background. Currents evoked by ATP are reliable and of high amplitude. |
|---|---|
| Gender: | Female |
| CRISPR: | No |
| Production details: | When the cell culture reaches 90% confluence, the cells need to be passaged. So, cell culture medium is removed, wash once with PBS, and then treated with enough but minimun amount of 0.05% trypsin for 2 min, inside a 37C and 5% CO2 incubator. Trypsin activity is stop by addition of 10 times volume of complete cell culture medium. Aliquots of cell culture (1×10^6 cells in 1 ml frozen media per vial) can be frozen back for long time storage in liquid nitrogen. Number of cell passages can be minized by subculturing the cells once a week, with the right amount of cells according to the size of the culture dish or flask used. |
| Parental cell line: | HEK293 |
| Initial handling information: | The vial containing the frozen cells is warm up in a bath. Cells are resuspended before the storage medium containing the cells are completely thawed, with 1 ml of culture cell media. The resuspension is transferred to a 15 ml tube and 8 ml of cell culture media is added and gently resuspended again. The cell suspension is centriguged at 1000 rpm for 5 min and the superntant discarded. The cells pellet is resuspended in 2 ml of cell culture media and seed it in a T-75 flask with 20 ml final volume of media. The day after, cell culture media is removed to eliminate death cells and replaced by a fresh cell culture media. If the culture is confluent, then it needs to be passaged. So, cells will be treated with 0.05% trypsin for 2 min inside a 37C and 5% CO2 incubator to dettach the cells from the botom of the flask. A gentle blow of the flask will warranty all the cells to be dettached. Check it out under the microscope. |
|---|---|
| Format: | Frozen |
| Shipping conditions: | Dry ice |
| Growth medium: | DMEM medium with 10% FBS, 1% Penicillin/Streptomycin, and G-418 (0.6 mg/ml). |
| Temperature: | 37° C |
| Atmosphere: | 5% CO2 |
| Storage medium: | Human P2X3 receptor channel |
| Recommended controls: | Wildtype HEK293 cells |
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