BICR 6 Cell Line

Adherent keratinocyte cell line derived from a squamous cell carcinoma of the hypopharynx of a Caucasian male.

Contributors

Inventor Institute
Eric Kenneth Parkinson Cancer Research UK Glasgow: The Beatson Institute
Cat. #: 152848
Tool sub type: 10XUAS IVS p10
Unit size: 1×10^6 cells / vial
Cancer types: Head and neck cancer;Head and neck cancer
Organism: Human
Tissue: Hypopharynx
Gender: Male
Model: Tumourigenic cell line
Growth properties: Adherent
Cancer Types In Detail: Hypopharynx squamous cell carcinoma;Hypopharynx squamous cell carcinoma
Alternate name: bicr6; BICR-6; BICR6; Beatson Institute for Cancer Research 6; HNSCC; SCC-HN
Product description: BICR 6 is an adherent keratinocyte cell line derived from a squamous cell carcinoma of the hypopharynx of a Caucasian male. The tumour stage (TNM) of the culture is T4 N1 M0. Keratin and involucrin markers are present. Epidermal Growth Factor Receptor (EGFR) is also present. Known mutations: p53, p16 and p14ARF. These cells are tumourigenic in athymic mice.
Gender: Male
CRISPR: Yes
Conditional: Yes
Additional notes: STR-PCR Data: Amelogenin: X, Y CSF1PO: 10 D13S317: 14 D16S539: 11 D5S818: 12 D7S820: 10 THO1: 6 TPOX: 9,11 vWA: 16,17
Cellosaurus ID: CVCL_2314
disease: Cancer;Cancer
Format: Frozen
Shipping conditions: Dry ice
Growth medium: DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) + 0.4 µg/ml Hydrocortisone.
Subculture routine: Split sub-confluent cultures (70-80%) 1:10 – 1:50 using 0.05% trypsin/EDTA; 8% CO₂; 37°C. Suggested seeding density of 2 x 10,000 cells/cm². Culture cells on a feeder layer of lethally-irradiated or mitomycin C-treated 3T3 Swiss Albino cells. Feeder layers are prepared in the flasks at least 24 hours in advance of being required. Where frozen stocks of treated 3T3 Swiss Albino cells have been prepared, an ampoule is thawed in a 37°C water bath and the contents quickly transferred to a 15ml centrifuge tube. DMEM medium is added drop wise to 5ml. Cells are centrifuged at 150 x g for 5 minutes at Room Temperature. Cells are resuspended in 5ml of medium. Cells are counted and added to flasks containing the correct BICR growth medium at 1-3 x 10^4 cells/cm². Please see detailed protocol within the Product Datasheet below.
Temperature: 37°C
Atmosphere: 5% CO2
Mycoplasma free: Yes
References:

Oh et al. 2021. Nat Commun. 12(1). PMID: 34389714.

Leonard et al. 2019. Oral Oncol. 95:35-42. PMID: 31345392.

Agochiya et al. 1999. Oncogene. 18(41):5646-5653. PMID: 10523844.

Edington et al. 1995. Mol Carcinog. 13(4):254-265. PMID: 7646764

Burns et al. 1993. Br J Cancer. 67(6):1274-1284. PMID: 8390283

Images

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Characteristics of the BICR human head and neck squamous cell carcinoma (HNSCC) cell line panel, including tissue of origin, tumour (T), node (N), and metastasis (M) stage, and TP53 mutation status.

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