Monoclonal antibody which labels all secretory cells within the zebrafish intestinal epithelium. Subsequent studies have reported that the 2F11 monoclonal antibody recognises Annexin A4 (Anxa4) in zebrafish.
| Inventor | Institute |
|---|---|
| Cecile Wright-Crosnier ; Julian Lewis ; Linda Ariza-McNaughton ; Neil Vargesson | Cancer Research UK, London Research Institute: Lincoln's Inn Fields |
| Cat. #: | 151483 |
|---|---|
| Tool sub type: | Primary antibody |
| Unit size: | 100 ug |
| Research Fields: | Cell biology;Cell signaling and signal transduction;Developmental biology;Genetics;Stem cell biology;Tags and cell markers |
| Application: | IHC ; ELISA ; IHC ; IF ; IP ; WB |
| Target: | Zebrafish gut secretory cell epitopes |
| Reactivity: | Zebrafish |
| Clone: | FIS 2F11/2 |
| Host: | Mouse |
| Class: | Monoclonal |
| Product description: | Anti-Zebrafish gut secretory cell epitopes [FIS 2F11/2] is a monoclonal antibody that labels all secretory cells within the zebrafish intestinal epithelium. Subsequent studies have reported that the 2F11 monoclonal antibody recognises Annexin A4 (Anxa4) in zebrafish. |
|---|---|
| Conjugation: | Unconjugated |
| Isotype: | IgG1 |
| Molecular weight: | 170 kDa, 120 kDa, 90 kDa and 35 kDa |
| Immunogen: | Lysate of zebrafish intestine |
| Myeloma used: | Sp2/0-Ag14 |
| Target background: | The transparency of the juvenile zebrafish and its genetic advantages make it an attractive model for the study of intestinal differentiation and renewal. This antibody labels all the gut secretory cells of the zebrafish intestinal epithelium, both mucous and enteroendocrine, corresponding to the class of cells that are lost in MathI mouse mutants. The antibody also labels hepatopancreatic ducts, intrahepatic bile ducts and enteroendocrine. Anti-2F11 provides an early marker to distinguish cell types and cell polarity in the zebrafish gut. |
|---|
| Format: | Liquid |
|---|---|
| Concentration: | 1 mg/ml |
| Storage buffer: | PBS with 0.02% azide |
| Storage conditions: | Store at -20° C frozen. Avoid repeated freeze / thaw cycles |
| Shipping conditions: | Dry ice |
| References: |
Nissim et al. 2019. Nat Genet. 51(9):1308-1314. PMID: 31406347. Gao et al. 2019. J Mol Cell Biol. 11(6):448-462. PMID: 30428031. Murdoch et al. 2019. PLoS Pathog. 15(3):e1007381. PMID: 30845179. Hong et al. 2018. Mol Med Rep. 17(2):2837-2844. PMID: 29257268. Hong et al. 2018. Mol Med Rep. 17(2):2837-2844. PMID: 29257268. Freudenblum et al. 2018. Development. 145(3):. PMID: 29386244. Freudenblum et al. 2018. Development. 145(3):. PMID: 29386244. Tom et al. 2015. Sci Rep. 5:14384. PMID: 26399509. Kimmel et al. 2015. Sci Rep. 5:14241. PMID: 26384018. Wang et al. 2015. Dev Dyn. 244(6):724-35. PMID: 25773748. Ningappa et al. 2015. PLoS One. 10(9):e0138381. PMID: 26379158. Flasse et al. 2013. Dev Biol. 376(2):187-97. PMID: 23352790. Palha et al. 2013. PLoS Pathog. 9(9):e1003619. PMID: 24039582. Garnaas et al. 2012. Dev Biol. 372(2):178-89. PMID: 22982668. Manfroid et al. 2012. Dev Biol. 366(2):268-78. PMID: 22537488. Ninov et al. 2012. Development. 139(9):1557-67. PMID: 22492351. Poulain et al. 2011. Development. 138(16):3557-68. PMID: 21771809. Shin et al. 2011. Development. 138(7):1339-48. PMID: 21385764. Matthews et al. 2008. Dev Dyn. 237(1):124-31. PMID: 18095340. Transcription factor onecut3 regulates intrahepatic biliary development in zebrafish. Dong et al. 2007. Nat Genet. 39(3):397-402. PMID: 17259985. Fgf10 regulates hepatopancreatic ductal system patterning and differentiation. Bates et al. 2006. Dev Biol. 297(2):374-86. PMID: 16781702. Distinct signals from the microbiota promote different aspects of zebrafish gut differentiation. Crosnier et al. 2005. Development. 132(5):1093-104. PMID: 15689380. Delta-Notch signalling controls commitment to a secretory fate in the zebrafish intestine. |
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