Cat. #154471
Anti-Sun1 [BBmSun1 X12.11]
Cat. #: 154471
Sub-type: Primary antibody
Unit size: 100 ug
Availability: 10-12 weeks
Target: Sun1 type II membrane protein
Class: Monoclonal
Application: IHC ; IF ; WB
Reactivity: Mouse
Host: Mouse
£300.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Brian Burke
Institute: A*STAR Accelerate Technologies Pte Ltd
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: Anti-Sun1 [BBmSun1 X12.11]
- Alternate name: Sun1
- Research fields: Genetics
- Tool sub type: Primary antibody
- Class: Monoclonal
- Purpose: Marker
- Conjugation: Unconjugated
- Molecular weight: Mouse 102 kDa; existence of multiple short isoforms
- Reactivity: Mouse
- Host: Mouse
- Application: IHC ; IF ; WB
- Description: SUN (Sad1, UNC-84) domain-containing proteins are type II membrane proteins that span the inner nuclear membrane. SUN proteins interact with lamins and other proteins in the nucleoplasm and with KASH (Klarsicht, ANC-1, and Syne homology) domain-containing proteins in the perinuclear space. Since the KASH proteins span the outer nuclear membrane and interact with components of the cytoskeleton, the SUN-KASH complex, also known as the LINC complex (LInker of the Nucleoskeleton and Cytoskeleton), provides a physical link between the nuclear interior and the cytoskeleton. The LINC complex is involved in diverse cellular functions including nuclear architecture, nuclear anchorage and migration, meiotic chromosome movement and cytoskeletal organization. Not surprisingly, aberrations in the LINC complex have been associated with number of human genetic diseases, including muscular dystrophy, cerebellar ataxia, hearing loss and infertility (1-3). Sun1 is one of at least five mammalian SUN protein family members and is ubiquitously expressed. Of note, Sun1 undergoes extensive alternative splicing. Human and mouse tissues express simultaneously several isoforms with the presence and prevalence of specific isoforms being tissue dependent (4,5).
- Immunogen: Bacterially expressed fragment (close to the Sun domain) of mouse Sun1. The details of the immunogen is proprietary information. The antibody should detect all the known isoforms.
- Isotype: IgG2b
- Recommended controls: C2C12, mouse fibroblast
Target Details
- Target: Sun1 type II membrane protein
- Molecular weight: Mouse 102 kDa; existence of multiple short isoforms
- Tissue cell line specificity: Mouse
- Target background: SUN (Sad1, UNC-84) domain-containing proteins are type II membrane proteins that span the inner nuclear membrane. SUN proteins interact with lamins and other proteins in the nucleoplasm and with KASH (Klarsicht, ANC-1, and Syne homology) domain-containing proteins in the perinuclear space. Since the KASH proteins span the outer nuclear membrane and interact with components of the cytoskeleton, the SUN-KASH complex, also known as the LINC complex (LInker of the Nucleoskeleton and Cytoskeleton)...
Applications
- Application: IHC ; IF ; WB
Handling
- Format: Liquid
- Unit size: 100 ug
- Shipping conditions: Dry ice
References
- Nishioka et al. 2016. Nucleus. 7(6):572-584. PMID: 27858498.
- Horn et al. 2014. Curr Top Dev Biol. 109:287-321. PMID: 24947240.
- Rothballer et al. 2013. Nucleus. 4(1):29-36. PMID: 23324460.
- Gb et al. 2011. Commun Integr Biol. 4(4):440-2. PMID: 21966565.
- Crisp et al. 2006. J Cell Biol. 172(1):41-53. PMID: 16380439.