Anti-SIGLEC8 [7C9]

The phosphorylated ITIM motif binds to the SH2 domain of PTPN6/SHP-1.

Contributors

Inventor Institute
Paul Crocker University of Dundee
Cat. #: 151590
Unit size: 100 ug
Research Fields: Cell biology;Cell signaling and signal transduction;Immunology
Application: ELISA ; FACS ; IP
Target: SIGLEC8
Reactivity: Human
Clone: 7C9
Host: Mouse
Class: Monoclonal
Product description: SIGLEC8 is a putative adhesion molecule that mediates sialic-acid dependent binding to cells. It preferentially binds to alpha2,3-linked sialic acid. and also binds to alpha2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. SIGLEC8 is expressed specifically on eosinophils. The protein contains 1 copy of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in downmodulation of cellular responses. The phosphorylated ITIM motif binds to the SH2 domain of PTPN6/SHP-1. The SIGLEC8 gene belongs to the immunoglobulin superfamily.
Conjugation: Unconjugated
Isotype: IgG1 kappa
Immunogen: Siglec-8-Fc protein, containing entire extracellular region of Human Siglec-8 fused with the Fc region of human IgG1
Myeloma used: Sp2/0-Ag14
Target background: SIGLEC8 is a putative adhesion molecule that mediates sialic-acid dependent binding to cells. It preferentially binds to alpha2,3-linked sialic acid. and also binds to alpha2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. SIGLEC8 is expressed specifically on eosinophils. The protein contains 1 copy of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in downmodulation of cellular responses. The phosphorylated ITIM motif binds to the SH2 domain of PTPN6/SHP-1. The SIGLEC8 gene belongs to the immunoglobulin superfamily.
Format: Liquid
Concentration: 1 mg/ml
Storage buffer: DMEM + 10% Fetal Clone I + 1% penecillin/streptomycin
Storage conditions: -15° C to -25° C
Shipping conditions: Dry ice
References:

Floyd et al. 2000. J Biol Chem. 275:861-6 PMID: 10625619

Nguyen et al. 2006. Proc Natl Acad Sci U S A. 103(20):7765-70. PMID: 16682635.

Images

View Gallery
Adapted from Floyd H; Ni J; Cornish AL; Zeng Z; Liu D; Carter KC; Steel J; Crocker PR. Siglec-8. A novel eosinophil-specific member of the immunoglobulin superfamily. J Biol Chem. 2000 Jan 14;275(2)

Related Tools for Antibodies

Cat. # Tool Name
151024 Anti-Hairy [1/24] Key Info View Tool
151025 Anti-Vascular endothelium [10] Key Info View Tool
151026 Anti-FCGR1 [10.1] mAb Key Info View Tool
151029 Anti-HSVICP8 [11E2] Key Info View Tool
151020 Anti-CyclinB1 [V143.1] Key Info View Tool

Tool enquiry

Please ensure you use your organisation email address rather than personal where possible, as this helps us locate your organisation in our system faster.

Please note we may take up to three days to respond to your enquiry.

CancerTools.org uses the contact information provided to respond to you about our research tools and service. For more information please review our privacy policy.