#151671

Anti-S100a9 [2B10]

Cat. #151671

Anti-S100a9 [2B10]

Cat. #: 151671

Sub-type: Primary antibody

Unit size: 100 ug

Availability: 3-4 weeks

Target: S100a9

Class: Monoclonal

Application: IF ; FACS ; IHC ; IF ; IP ; WB

Reactivity: Mouse

Host: Rat

£300.00

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Inventor: Nancy Hogg

Institute: Cancer Research UK, London Research Institute: Lincoln's Inn Fields

Tool Details
Target Details
Applications
Handling
References

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

  • Name: Anti-S100a9 [2B10]
  • Research fields: Cancer;Cell signaling and signal transduction;Immunology
  • Clone: 2B10
  • Tool sub type: Primary antibody
  • Class: Monoclonal
  • Conjugation: Unconjugated
  • Reactivity: Mouse
  • Host: Rat
  • Application: IF ; FACS ; IHC ; IF ; IP ; WB
  • Description: The mAb is specific for mouse S100a9 protein that forms a heterodimer with S100a8 and constitutes 45% of the neutrophil cytosolic protein. The mAb stains neutrophils strongly and monocytes weakly.
  • Immunogen: Recombinant full-length murine S100a9
  • Isotype: IgG1 kappa
  • Myeloma used: Sp2/0-Ag14
  • Recommended controls: Neutrophils

Target Details

  • Target: S100a9
  • Tissue cell line specificity: Neutrophils
  • Target background: The mAb is specific for mouse S100a9 protein that forms a heterodimer with S100a8 and constitutes 45% of the neutrophil cytosolic protein. The mAb stains neutrophils strongly and monocytes weakly.

Applications

  • Application: IF ; FACS ; IHC ; IF ; IP ; WB

Handling

  • Format: Liquid
  • Concentration: 1 mg/ml
  • Unit size: 100 ug
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: -15° C to -25° C
  • Shipping conditions: Shipping at 4° C

References

  • Colom et al. 2015. Immunity. 42(6):1075-86. PMID: 26047922.
  • Leukotriene B4-Neutrophil Elastase Axis Drives Neutrophil Reverse Transendothelial Cell Migration In Vivo.
  • Chen et al. 2015. Mucosal Immunol. 8(2):390-402. PMID: 25160819.
  • IL-23 activates innate lymphoid cells to promote neonatal intestinal pathology.
  • Pericytes support neutrophil subendothelial cell crawling and breaching of venular walls in vivo.
  • Voisin et al. 2010. Am J Pathol. 176(1):482-95. PMID: 20008148.
  • Venular basement membranes ubiquitously express matrix protein low-expression regions: characterization in multiple tissues and remodeling during inflammation.
  • Young et al. 2007. Br J Pharmacol. 151(5):628-37. PMID: 17471175.
  • Role of neutrophil elastase in LTB4-induced neutrophil transmigration in vivo assessed with a specific inhibitor and neutrophil elastase deficient mice.
  • Hobbs et al. 2003. Mol Cell Biol. 23(7):2564-76. PMID: 12640137.
  • Myeloid cell function in MRP-14 (S100A9) null mice.