Anti-Plx [AZ44]

Polo-like kinase (Plx) is a mitotic regulator conserved from yeasts to humans. Many key cell cycle regulators such as p53, cdc25, cyclin B and the Anaphase Promoting Complex are directly targeted by Plx.The Anaphase promoting complex is responsible for the destruction of cyclins and other proteins involved with mitosis.

Contributors

Inventor Institute
Julian Gannon Cancer Research UK, London Research Institute: Clare Hall Laboratories
Cat. #: 151301
Tool sub type: Primary antibody
Unit size: 100 ug
Research Fields: Cell biology
Application: WB ; ELISA ; WB
Target: Polo-like-kinase protein (Plk)
Reactivity: Xenopus laevis
Clone: AZ44
Host: Mouse
Class: Monoclonal
Alternate name: Polo Like Kinase 1; Serine/Threonine-Protein Kinase 13; EC 2.7.11.21; STPK13; PLK-1; Cell Cycle Regulated Protein Kinase; Polo Like Kinase; EC 2.7.11
Product description: Polo-like kinase (Plx) is a mitotic regulator conserved from yeasts to humans. Many key cell cycle regulators such as p53, cdc25, cyclin B and the Anaphase Promoting Complex are directly targeted by Plx.The Anaphase promoting complex is responsible for the destruction of cyclins and other proteins involved with mitosis.
Conjugation: Unconjugated
Isotype: IgG1 kappa
Immunogen: Baculo expressed Polo-like-kinase from Xenopus laevis
Myeloma used: Sp2/0-Ag14
Target background: Polo-like kinase (Plx) is a mitotic regulator conserved from yeasts to humans. Many key cell cycle regulators such as p53, cdc25, cyclin B and the Anaphase Promoting Complex are directly targeted by Plx.The Anaphase promoting complex is responsible for the destruction of cyclins and other proteins involved with mitosis.
Format: Liquid
Concentration: 1 mg/ml
Storage buffer: PBS with 0.02% azide
Storage conditions: -15° C to -25° C
Shipping conditions: Dry ice
References:

Esmaili et al. 2010. Cell Cycle. 9(19):3913-20. PMID: 20935510.

Wu et al. 2009. Nat Cell Biol. 11(5):644-51. PMID: 19396163.

Images

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mRNA encoding either Xenopus ?-globin (x-?globin); Flag-tagged human Aven-WT or Flag-tagged Aven-NES282-292 were added to cycling Xenopus egg extracts to drive protein expression. Aliquots of extracts were then taken at the indicated times and immunoblotted with anti-phospho polo-like kinase-1 (pPlx; upper panels) and antiphospho Tyr15-Cdc2 (Cdc2 pY15; lower panels) antibodies. Adapted from Esmaili et al. 2010. Cell Cycle. 9(19)

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