#152689

Anti-PLAP [H17E2]

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Cat. #152689

Anti-PLAP [H17E2]

Cat. #: 152689

Sub-type: Primary antibody

Unit size: 100 ug

Availability: 3-4 weeks

Target: Placenta-specific isozyme of Alkaline Phosphatase (PLAP)

Class: Monoclonal

Application: ELISA ; IHC

Reactivity: Human

Host: Mouse

£300.00

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Inventor: Walter Bodmer

Institute: Cancer Research UK, London Research Institute: Lincoln's Inn Fields

Tool Details
Target Details
Applications
Handling
References

Tool Details

*FOR RESEARCH USE ONLY

  • Name: Anti-PLAP [H17E2]
  • Research fields: Cancer;Cell biology
  • Clone: H17E2
  • Tool sub type: Primary antibody
  • Class: Monoclonal
  • Conjugation: Unconjugated
  • Molecular weight: 67 kDa
  • Strain: Balb/c
  • Reactivity: Human
  • Host: Mouse
  • Application: ELISA ; IHC
  • Description: Monoclonal antibody detects PLAP protein, used as a marker for various cancers, including ovarian and testicular. Background and Research Application Human placental alkaline phosphatase (PLAP) is a membrane bound glycosylated phosphodiesterase normally synthesised by syncytiotrophoblast from the 12th week of pregnancy. Since its identification as an oncofoetal antigen, it has been found to be expressed by malignant tumours of germ cell and non-germ cell origin. The detection of alkaline phosphatase in serum is a marker for ovarian and testicular cancer. The placental-specific isozyme of Alkaline Phosphatase (PLAP), also referred to as the heat-stable form, is found in trophoblast cells of normal human mature placenta, seminomas of testis and ovarian carcinomas. It is closely related to the intestinal form of the enzyme as well as to the placental-like form. Anti-PLAP was created for use in immunoscintigraphy or therapy. The detection of alkaline phosphatase in serum is a marker for ovarian and testicular cancer. The antibody detects both Regan and Nagao isoenzymes.
  • Immunogen: Placental membranes prepared from fresh term placenta
  • Immunogen uniprot id: P05187
  • Isotype: IgG1
  • Myeloma used: P3/NS1/1-Ag4.1

Target Details

  • Target: Placenta-specific isozyme of Alkaline Phosphatase (PLAP)
  • Molecular weight: 67 kDa
  • Target background: Monoclonal antibody detects PLAP protein, used as a marker for various cancers, including ovarian and testicular. Background and Research Application Human placental alkaline phosphatase (PLAP) is a membrane bound glycosylated phosphodiesterase normally synthesised by syncytiotrophoblast from the 12th week of pregnancy. Since its identification as an oncofoetal antigen, it has been found to be expressed by malignant tumours of germ cell and non-germ cell origin. The detection of alkaline phosphatase in serum is a marker for ovarian and testicular cancer. The placental-specific isozyme of Alkaline Phosphatase (PLAP), also referred to as the heat-stable form, is found in trophoblast cells of normal human mature placenta, seminomas of testis and ovarian carcinomas. It is closely related to the intestinal form of the enzyme as well as to the placental-like form. Anti-PLAP was created for use in immunoscintigraphy or therapy. The detection of alkaline phosphatase in serum is a marker for ovarian and testicular cancer. The antibody detects both Regan and Nagao isoenzymes.

Applications

  • Application: ELISA ; IHC

Handling

  • Format: Liquid
  • Concentration: 1 mg/ml
  • Unit size: 100 ug
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: Store at -20° C frozen. Avoid repeated freeze / thaw cycles
  • Shipping conditions: Shipping at 4° C

References

  • Fitzgerald et al. 2018. Am J Reprod Immunol. 80(1):e12860. PMID: 29726582.
  • Streicher et al. 2017. Sci Rep. 7(1):6460. PMID: 28744019.
  • Multicolor flow cytometry and nanoparticle tracking analysis of extracellular vesicles in the plasma of normal pregnant and pre-eclamptic women.
  • Dragovic et al. 2013. Biol Reprod. 89(6):151. PMID: 24227753.
  • Tucker et al. 1985. Br J Cancer. 51(5):631-9. PMID: 2986664.
  • Epenetos et al. 1985. Br J Cancer. 51(5):641-4. PMID: 3994908.
  • Serum marker potential of placental alkaline phosphatase-like activity in testicular germ cell tumours evaluated by H17E2 monoclonal antibody assay.
  • Monoclonal antibody assay of serum placental alkaline phosphatase in the monitoring of testicular tumours.
  • Travers et al. 1984. Int J Cancer. 33(5):633-41. PMID: 6202646.
  • Preparation and characterization of monoclonal antibodies against placental alkaline phosphatase and other human trophoblast-associated determinants.
  • Epenetos et al. 1984. Br J Cancer. 49(1):11-5. PMID: 6362705.
  • An immunohistological study of testicular germ cell tumours using two different monoclonal antibodies against placental alkaline phosphatase.