Cat. #151131
Anti-PKCa [MC5]
Cat. #: 151131
Sub-type: Primary antibody
Unit size: 100 ug
Availability: 3-5 days
Target: Protein Kinase C alpha
Class: Monoclonal
Application: ELISA ; FACS ; IHC ; IF ; IP ; RIA ; WB
Reactivity: Bovine
Host: Mouse
£300.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Peter Parker
Institute: Cancer Research UK, London Research Institute: Lincoln's Inn Fields
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: Anti-PKCa [MC5]
- Alternate name: CD1c Molecule; CD1C Antigen; C Polypeptide; CD1c Antigen; Differentiation Antigen CD1-Alpha-3; Cortical Thymocyte Antigen; CD1C; BDCA1; CD1A; CD1; R7
- Research fields: Cancer;Tissue-specific biology;Cell signaling and signal transduction
- Clone: MC5
- Tool sub type: Primary antibody
- Class: Monoclonal
- Conjugation: Unconjugated
- Molecular weight: 80 kDa
- Strain: Balb/c
- Reactivity: Bovine
- Host: Mouse
- Application: ELISA ; FACS ; IHC ; IF ; IP ; RIA ; WB
- Description: MC5 blocks partial proteolysis of PKC by trypsin in vitro. Fab fragment inhibits phorbol-ester-induced down-regulation of PKC in a rodent gloma cell line.
- Immunogen: Purified bovine brain protein kinase C
- Isotype: IgG2a
- Myeloma used: P3X63Ag8
Target Details
- Target: Protein Kinase C alpha
- Molecular weight: 80 kDa
- Target background: Protein Kinase C is a family of serine/threonine protein kinases that are activated by Ca2+, diacylglycerol and membrane phospholipids (phosphatidylserine).
Applications
- Application: ELISA ; FACS ; IHC ; IF ; IP ; RIA ; WB
Handling
- Format: Liquid
- Concentration: 1 mg/ml
- Unit size: 100 ug
- Storage buffer: PBS with 0.02% azide
- Storage conditions: -15° C to -25° C
- Shipping conditions: Shipping at 4° C
References
- Kim et al. 2008. Nature. 452(7186):478-82. PMID: 18368118.
- Molecular identification of a retinal cell type that responds to upward motion.
- Ng et al. 1999. EMBO J. 18(14):3909-23. PMID: 10406796.
- PKCalpha regulates beta1 integrin-dependent cell motility through association and control of integrin traffic.
- Young et al. 1988. Eur J Biochem. 173(1):247-52. PMID: 2451608.
- A monoclonal antibody recognising the site of limited proteolysis of protein kinase C. Inhibition of down-regulation in vivo.
