A notable feature of metazoan cells is their morphological plasticity associated with acquisition of a range of complex activities. Implementation of numerous specialized functions during differentiation is dependent upon the rearrangement and transformation of organelles, including the cell nucleus.
| Institute |
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| A*STAR Accelerate Technologies Pte Ltd |
| Cat. #: | 153480 |
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| Tool sub type: | Primary antibody |
| Unit size: | 100 ug |
| Research Fields: | Developmental biology;Genetics |
| Application: | IF ; WB |
| Target: | Nesprin-1 |
| Reactivity: | Mouse ; Rat |
| Clone: | 9F10 |
| Host: | Mouse |
| Class: | Monoclonal |
| Alternate name: | Nesp1 antibody, Nesprin-1 antibody, Nuclear envelope spectrin repeat protein 1 antibody |
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| Product description: | A notable feature of metazoan cells is their morphological plasticity associated with acquisition of a range of complex activities. Implementation of numerous specialized functions during differentiation is dependent upon the rearrangement and transformation of organelles, including the cell nucleus. Crucial to many cell specific changes in nuclear localization and organization is establishment of connections between the nucleus and cytoskeleton. Such connections are formed in part by KASH-do… |
| Conjugation: | Unconjugated |
| Isotype: | IgG2b kappa |
| Immunogen: | GST fused to residues 8,165-8,473 of mouse Nesp1 |
| Myeloma used: | Sp2/0-Ag14 |
| Target background: | A notable feature of metazoan cells is their morphological plasticity associated with acquisition of a range of complex activities. Implementation of numerous specialized functions during differentiation is dependent upon the rearrangement and transformation of organelles, including the cell nucleus. Crucial to many cell specific changes in nuclear localization and organization is establishment of connections between the nucleus and cytoskeleton. Such connections are formed in part by KASH-do… |
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| Format: | Liquid |
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| Concentration: | 1 mg/ml |
| Storage buffer: | PBS with 0.02% azide |
| Storage conditions: | -15° C to -25° C |
| Shipping conditions: | Dry ice |
HEK293 cells transiently expressing GFP alone; GFP fused to a small Nesp2 isoform (Nesp2a1) or to a Nesp1 isoform (Nesp1a). The small Nesp1 isoform contains the ~300 residue Nesp1 polypeptide that was employed in the immunogen. Total cell lysates were analyzed by western blot employing either a rabbit anti-GFP or culture supernatant from the anti-Nesp1 hybridoma clone (9F10). The antibody is specific for Nesp1.

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