Antibody Amp4/42 recognises the ring-opened structure that forms when DNA is alkylated by the anti-cancer drug melphalan and is then exposed to alkali
| Inventor | Institute |
|---|---|
| Michael Tilby | The Institute of Cancer Research; Newcastle University |
| Cat. #: | 151465 |
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| Tool sub type: | Primary antibody |
| Unit size: | 100 ug |
| Research Fields: | Cancer;Genetics |
| Application: | ELISA ; IF |
| Target: | Melphalan |
| Reactivity: | Human ; Mouse |
| Clone: | Amp4/42 |
| Host: | Rat |
| Class: | Monoclonal |
| Product description: | Antibody Amp4/42 recognises the ring-opened structure that forms when DNA is alkylated by the anti-cancer drug melphalan and is then exposed to alkali |
|---|---|
| Conjugation: | Unconjugated |
| Isotype: | IgG2b |
| Myeloma used: | Y3.AG.1.2.3 |
| Target background: | The alkali causes guanine bases alkylated at the N7 position to undergo a so-called ring-opening reaction and Amp4/42 appears to recognise this structure, although recognition may be influenced by the neighbouring DNA sequence. The products of the ring-opening reaction are more resistant to depurination reactions than the original guanine adducts. This antibody was developed to improve detection of melphalan-DNA adducts through its ability to recognise the stabilised adduct. The Amp4/42 clone did not bind to alkali-treated control DNA or to DNA that had been alkylated with melphalan but not exposed to alkali. This antibody can be used for quantification of melphalan adducts in purified DNA by ELISA and for immunofluorescent detection of melphalan-DNA adducts in single cells. |
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| Format: | Liquid |
|---|---|
| Concentration: | 1 mg/ml |
| Storage buffer: | PBS with 0.02% azide |
| Storage conditions: | -15° C to -25° C |
| Shipping conditions: | Dry ice |
| References: |
Frank AJ and Tilby MJ. Exp Cell Res. 2003 Feb 15 283(2):127-34.PMID: 12581733 Buschfort-Papewalis et al. 2002. Blood. 100(3):845-53. PMID: 12130494. Down-regulation of DNA repair in human CD34(+) progenitor cells corresponds to increased drug sensitivity and apoptotic response. McCartney et al. 2001. Chem Res Toxicol. 14(1):71-81. PMID: 11170510. McCartney et al. 2001. Chem Res Toxicol. 14(1):71-81. PMID: 11170510. Antibody recognition of melphalan adducts characterized using immobilized DNA: enhanced alkylation of G-Rich regions in cells compared to in vitro. Tilby et al. 1998. Chem Res Toxicol. 11(10):1162-8. PMID: 9778312. A monoFn derivative of melphalan: preparation, DNA alkylation products, and determination of the specificity of monoclonal antibodies that recognize melphalan-DNA adducts. Tilby et al. 1995. Carcinogenesis. 16(8):1895-901. PMID: 7634420. A monoclonal antibody that recognizes alkali-stabilized melphalan-DNA adducts and its application in immunofluorescence microscopy. |
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