Cat. #151022
Anti-IL12 [1-1A4]
Cat. #: 151022
Sub-type: Primary antibody
Unit size: 100 ug
Availability: 3-4 weeks
Target: Interleukin-12 (IL12) p40 subunit
Class: Monoclonal
Application: IHC ; ELISA ; IHC ; RIA ; WB
Reactivity: Human
Host: Mouse
£300.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Rosemonde Banks
Institute: University of Leeds
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: Anti-IL12 [1-1A4]
- Alternate name: Interleukin 12; Cytotoxic Lymphocyte Maturation Factor 4 KDa Subunit; NK Cell Stimulatory Factor Chain; Interleukin-12 Beta Chain; IL-12 Subunit P4; CLMF P4; NKSF2; Natural Killer Cell Stimulatory Factor; IL12; Subunit P4; Interleukin 12; CLMF2; IMD29; NKSF; P4
- Research fields: Immunology
- Clone: 1-1A4
- Tool sub type: Primary antibody
- Class: Monoclonal
- Conjugation: Unconjugated
- Reactivity: Human
- Host: Mouse
- Application: IHC ; ELISA ; IHC ; RIA ; WB
- Description: Monoclonal antibody which binds p40 subunit of interleukin-12.
- Immunogen: Recombinant human IL-12 produced in Baculovirus.
- Immunogen uniprot id: P29459
- Isotype: IgG1
- Myeloma used: Sp2/0-Ag14
Target Details
- Target: Interleukin-12 (IL12) p40 subunit
- Target background: IL-12 is a heterodimeric cytokine comprised of p35 and p40 subunits. IL-12 plays a central role in cell-mediated immunity, promoting the differentiation of CD4+ T cells to the Th1 subset and of CD8+ T cells into mature cytotoxic T lymphocytes (CTLs). Interleukin 12 (IL-12) is produced by dendritic cells, macrophages, neutrophils, and human B-lymphoblastoid cells in response to antigenic stimulation. IL-12 is a potent stimulator of Natural Killer cells. IL-12 therapy has been suggested as a method of enhancing cytocidal anti-tumour immune responses. This antibody recognises the p40 subunit of human IL-12 and binds to both the free subunit and the p35/p40 heterodimer. It reacts with both stimulated and unstimulated monocytes in immunohistology.
Applications
- Application: IHC ; ELISA ; IHC ; RIA ; WB
Handling
- Format: Liquid
- Concentration: 1 mg/ml
- Unit size: 100 ug
- Storage buffer: PBS with 0.02% azide
- Storage conditions: Store at -20° C frozen. Avoid repeated freeze / thaw cycles
- Shipping conditions: Shipping at 4° C
References
- Nagashima et al. 2008. Anticancer Res. 28(2B):1277-83. PMID: 18505066.
- Prognostic significance of the local expression of interleukin-12 in patients with advanced gastric cancer.
- Nakayama et al. 2004. Anticancer Res. 24(5C):3289-94. PMID: 15515423.
- Relationships between local and systemic expression of interleukin-12 and plasma levels of vascular endothelial growth factor in patients with gastric cancer.
- Carter et al. 1997. Hybridoma. 16(4):363-9. PMID: 9309427.
- Production and characterization of monoclonal antibodies to human interleukin-12.
