Anti-HLA-E [3D12]

MoAb was combined to examine HLA expression in placental tissues for all the known forms of the nonclassical HLA-E class I molecules that is important in the maternal immune response during pregnancy. Antibody 3D12 showed that HLA-E peptide binding was correlated with surface expression.

Contributors

Inventor Institute
Daniel Geraghty Fred Hutchinson Cancer Center
Cat. #: 161648
Tool sub type: Primary antibody
Research Fields: Developmental biology;Immunology
Application: FACS, IP, ELISA, Histology, No Western Blot.
Target: HLA-E class I complexes
Reactivity: Human
Clone: 3D12
Host: Mouse
Class: Monoclonal
Primary citation: lshitani et al. 2003. Journal of Immunology. 171(3):1376-84. PMID: 12874228
Product description: MoAb was combined to examine HLA expression in placental tissues for all the known forms of the nonclassical HLA-E class I molecules that is important in the maternal immune response during pregnancy. Antibody 3D12 showed that HLA-E peptide binding was correlated with surface expression.
Isotype: IgG1
Molecular weight: ~40kDa
Target background: Antibody is used to examine expression in placental tissues for all the known forms of the HLA-E nonclassical class I molecules.
Storage conditions: The antibody solution should be stored undiluted between 2° C and 8° C, and protected from prolonged exposure to light. Do not freeze.For immediate use, short term storage at 4° C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20° C or -80° C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided.
Shipping conditions: Dry ice
References:

lshitani et al. 2003. Journal of Immunology. 171(3):1376-84.PMID: 12874228

Lee et al. 1998. J. Immunol.?160(10):4951-60. PMID: 9590243

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