Anti-CD45 [HLe-1(2D1)]

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

• Name: Anti-CD45 [HLe-1(2D1)]
• Alternate name: Anti-PTPRC
• Research fields: Cell biology;Cell signaling and signal transduction;Immunology;Neurobiology;Stem cell biology;Tissue-specific biology
• Clone: HLe-1(2D1)
• Tool sub type: Primary antibody
• Class: Monoclonal
• Conjugation: Unconjugated
• Molecular weight: 180-220 kDa
• Strain: Balb/c
• Reactivity: Human
• Host: Mouse
• Application: FACS ; IHC ; IF
• Description: Monoclonal antibody capable of analysing subpopulations of hematopoietic and lymphoid cells, and the degree of T cell differentiation. It can be used to identify the effect of CD45 in immune function.
• Immunogen: Human peripheral blood mononuclear cells.
• Immunogen uniprot id: P08575
• Isotype: IgG1
• Myeloma used: P3/NS1/1-Ag4.1

Target Details

• Target: Protein tyrosine phosphatase, receptor type, C (PTPRC, CD45)
• Molecular weight: 180-220 kDa
• Target background: Anti-PTPRC (Hle-1) monoclonal antibody binds transmembrane protein tyrosine phosphatase receptor type C (PTPRC), also known as CD45 antigen or leukocyte common antigen (LCA). Anti-PTPRC can be used in a panel of several monoclonal antibodies to characterise tumours. This antibody was created to investigate the similarities between normal thymocyte subpopulations and leukaemia's of thymic origin (Thy-ALL), and aid with leukaemia diagnosis. PTPRC is present on all leukocytes. CD45 helps regulate T cell receptor-associated kinases and Janus kinases which transmit signals from cytokine receptors. This regulates the threshold of T cell antigen receptor (TCR) signalling through dephosphorylation of protein tyrosine kinases (e.g. Lck and Fyn). It is only expressed on nucleated hemopoietic cells (~10% of cell surface). CD45 phosphatase activity is required for efficient lymphocyte antigen receptor signal transduction. CD45 is a positive and negative regulator of Src family members, and can act as a JAK phosphatase, negatively regulating cytokine receptor activation (related to differentiation, proliferation and anti-viral immunity of hemopoietic cells).

Applications

• Application: FACS ; IHC ; IF

Handling

• Format: Liquid
• Concentration: 1 mg/ml
• Unit size: 100 ug
• Storage buffer: PBS with 0.02% azide
• Storage conditions: Store at -20° C frozen. Avoid repeated freeze / thaw cycles
• Shipping conditions: Dry ice

References

• Di Liberto et al. 2016. Clin Transl Gastroenterol. 7(7):e178. PMID: 27388423.
• Lee et al. 2004. Int Immunol. 16(8):1109-24. PMID: 15210650.
• Tchilian et al. 2001. J Immunol. 166(2):1308-13. PMID: 11145714.
• Bradstock et al. 1980. J Natl Cancer Inst. 65(1):33-42. PMID: 6993744.