MoAbs to CD34 will be valuable for future studies of canine hematopoiesis and preclinical studies concerning stem cell transplantation, and ex vivo progenitor cell expansionstudies of stem cell transplantation, progenitor cell expansion, gene therapy, and culture of canine dendritic cells
| Inventor | Institute |
|---|---|
| Richard Nash | Fred Hutchinson Cancer Center |
| Cat. #: | 161646 |
|---|---|
| Tool sub type: | Primary antibody |
| Research Fields: | Stem cell biology |
| Application: | Western Blotting, Flow Cytometry, CFU-GM assay, LTC-IC assay, ELISA |
| Target: | CD34 |
| Reactivity: | Human |
| Clone: | 2 e9 |
| Host: | Canine |
| Class: | Monoclonal |
| Primary citation: | McSweeney et al. 1998.Blood. 91(6):1977-86. PMID: 9490680 |
| Product description: | Good animal models in which to refin studies of CD34 cell fractions are limited. However, this MoAb detected 4% to 17% CD34 cells in murine Bone Marrow (BM) cells, somewhat higher than the 1% to 5% CD34 cells detected in human BM. Can be used to enrich canine progenitor cell populations that appear functionally and phenotypically similar to human CD34 cell |
|---|---|
| Isotype: | IgG-1 |
| Molecular weight: | 110kDa |
| Immunogen: | recombinant CD34 murine Ig fusion molecule (CD34-Ig) |
| Target background: | CD34 has previously been define as a marker of hematopoietic progenitors and is expressed on canine bone marrow (BM) progenitor cells in a manner analogous to that found in humans. |
|---|
| Storage conditions: | The antibody solution should be stored undiluted between 2° C and 8° C, and protected from prolonged exposure to light. Do not freeze.For immediate use, short term storage at 4° C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20° C or -80° C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. |
|---|---|
| Shipping conditions: | Dry ice |
| References: |
McSweeney et al. 1998.Blood. 91(6):1977-86.PMID:PMID: 9490680 Wayner et al. 1987.J Cell Biol.105 (1987): 1873.https://doi.org/10.1083/jcb.105.4.1873 |
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