#161646

Anti-CD34 [2E9]

Cat. #161646

Anti-CD34 [2E9]

Cat. #: 161646

Sub-type: Primary antibody

Target: CD34

Class: Monoclonal

Application: Western Blotting, Flow Cytometry, CFU-GM assay, LTC-IC assay, ELISA

Reactivity: Human

Host: Canine

£300.00

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Inventor: Richard Nash

Institute: Fred Hutchinson Cancer Research Center

Primary Citation: McSweeney et al. 1998.Blood. 91(6):1977-86. PMID: 9490680

Tool Details
Target Details
Applications
Handling
References

Tool Details

*FOR RESEARCH USE ONLY

  • Name: Anti-CD34 [2E9]
  • Clone: 2 e9
  • Tool sub type: Primary antibody
  • Class: Monoclonal
  • Molecular weight: 110kDa
  • Reactivity: Human
  • Host: Canine
  • Application: Western Blotting, Flow Cytometry, CFU-GM assay, LTC-IC assay, ELISA
  • Description: Good animal models in which to refin studies of CD34 cell fractions are limited. However, this MoAb detected 4% to 17% CD34 cells in murine Bone Marrow (BM) cells, somewhat higher than the 1% to 5% CD34 cells detected in human BM. Can be used to enrich canine progenitor cell populations that appear functionally and phenotypically similar to human CD34 cell
  • Immunogen: recombinant CD34 murine Ig fusion molecule (CD34-Ig)
  • Isotype: IgG-1

Target Details

  • Target: CD34
  • Molecular weight: 110kDa
  • Target background: CD34 has previously been define as a marker of hematopoietic progenitors and is expressed on canine bone marrow (BM) progenitor cells in a manner analogous to that found in humans.

Applications

  • Application: Western Blotting, Flow Cytometry, CFU-GM assay, LTC-IC assay, ELISA
  • Application notes: A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

Handling

  • Storage conditions: The antibody solution should be stored undiluted between 2° C and 8° C, and protected from prolonged exposure to light. Do not freeze.For immediate use, short term storage at 4° C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20° C or -80° C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided.

References

  • McSweeney et al. 1998.Blood. 91(6):1977-86.PMID:PMID: 9490680
  • Wayner et al. 1987.J Cell Biol.105 (1987): 1873.https://doi.org/10.1083/jcb.105.4.1873