#151407

Anti-BCL2 [bcl-2/124]

Cat. #151407

Anti-BCL2 [bcl-2/124]

Cat. #: 151407

Sub-type: Primary antibody

Unit size: 100 ug

Availability: 1-2 weeks

Target: Bcl2

Class: Monoclonal

Application: IHC ; WB

Reactivity: Human

Host: Mouse

£300.00

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Inventor: Jacqueline Cordell

Institute: University of Oxford

Primary Citation: Pezzella et al. 1990. Lancet. 336(8729):1510-1. PMID: 1979117

Tool Details
Target Details
Applications
Handling
References

Tool Details

*FOR RESEARCH USE ONLY

  • Name: Anti-BCL2 [bcl-2/124]
  • Clone: bcl-2/124
  • Tool sub type: Primary antibody
  • Class: Monoclonal
  • Conjugation: Unconjugated
  • Reactivity: Human
  • Host: Mouse
  • Disease: Breast Cancer
  • Application: IHC ; WB
  • Description: Monoclonal antibody which binds anti-apoptotic regulator BCL2, used to aid in differentiating between various lymphomas and as prognostic biomarker in various cancers.
  • Immunogen: Synthetic peptide of amino acids 41-54 of human bcl-2 oncoprotein
  • Immunogen uniprot id: P10415
  • Isotype: IgG1
  • Myeloma used: P3/NS1/1-Ag4.1

Target Details

  • Target: Bcl2
  • Target background: BCL2 is a member of the BCL-2 family of regulatory proteins, involved in apoptosis, which promotes cell survival and inhibits the role of pro-apoptotic proteins such as Bax and Bak. The pro-survival protein BCL2 derives its name from B-cell lymphoma 2. It is the second member of a range of proteins initially described in chromosomal translocations involving chromosomes 14 and 18 in follicular lymphomas. Damage to the Bcl-2 gene has been identified as a possible cause of schizophrenia and autoimmunity, a cause of cancers, including melanoma, breast, prostate, chronic lymphocytic leukaemia, and lung cancer. It also provides resistance to cancer treatments . This antibody was created to see if the BCL2 protein is a specific marker to the 14;18 chromosomal translocation in human follicular lymphoma. Previous studies claimed the BCL2 protein was not found in normal tissue but was present in all lymphomas where this specific translocation was observed. This antibody helped show the immunohistologic demonstration of BCL2 isn't specific for the presence of 14;18 translocation.

Applications

  • Application: IHC ; WB

Handling

  • Format: Liquid
  • Concentration: 1 mg/ml
  • Unit size: 100 ug
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: Store at -20° C frozen. Avoid repeated freeze / thaw cycles
  • Shipping conditions: Shipping at 4° C

References

  • Shimono et al. 2018. Am J Surg Pathol. 42(11):1466-1471. PMID: 30307409.
  • Nanni et al. 2018. Mol Cell Biol. 38(13):. PMID: 29685904.
  • Pricartet al. 2017. Medicine (Baltimore). 96(47):e8705. PMID: 29381956.
  • Chang et al. 2016. J Formos Med Assoc. :. PMID: 27773559.
  • Ishiguro et al. 2016. Hum Cell. :. PMID: 27613543.
  • Otali et al. 2016. Biotech Histochem. :1-18. PMID: 27149658.
  • Cortese et al. 2016. Neurology. :. PMID: 26843560.
  • Miltiades et al. 2015. Clin Cancer Res. :. PMID: 26700206.
  • Tajima et al. 2015. Int J Clin Exp Pathol. 8(3):3262-9. PMID: 26045850.
  • Pankova et al. 2015. Tumour Biol. :. PMID: 26456960.
  • Miyagi Maeshima et al. 2015. J Clin Exp Hematop. 55(1):7-11. PMID: 26106000.
  • Huo et al. 2015. Diagn Pathol. 10:80. PMID: 26112006.
  • Higashi et al. 2015. Leuk Lymphoma. :1-6. PMID: 25860238.
  • Navratil et al. 1995. J Clin Pathol. 48(1):18-21. PMID: 7706514.
  • Ashton-Key et al. 1995. Histopathology. 26(1):75-8. PMID: 7713486.
  • Piris et al. 1994. Br J Cancer. 69(2):337-41. PMID: 8297731.
  • Leek et al. 1994. Br J Cancer. 69(1):135-9. PMID: 8286195.
  • de Jong et al. 1994. Cancer Res. 54(1):256-60. PMID: 8261449.
  • Pezzella et al. 1993. N Engl J Med. 329(10):690-4. PMID: 8393963.
  • Doussis et al. 1993. Am J Clin Pathol. 99(6):663-7. PMID: 8322700.
  • Pezzella et al. 1993. Histopathology. 22(1):39-44. PMID: 8436340.
  • Gaulard et al. 1992. Am J Pathol. 140(5):1089-95. PMID: 1374590.
  • Pezzella et al. 1992. Br J Cancer. 65(1):87-9. PMID: 1733447.
  • Pezzella et al. 1990. Lancet. 336(8729):1510-1. PMID: 1979117.