Anti-5T4 [B3F1]

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

• Name: Anti-5T4 [B3F1]
• Alternate name: Trophoblast Glycoprotein; 5T4 Oncofetal Trophoblast Glycoprotein; Wnt-Activated Inhibitory Factor 1; 5T4 Oncotrophoblast Glycoprotein; 5T4 Oncofetal Antigen; WAIF1; M6P1; 5T4; 5T4AG
• Research fields: Cancer;Stem cell biology
• Clone: B3F1
• Tool sub type: Primary antibody
• Class: Monoclonal
• Conjugation: Unconjugated
• Molecular weight: 46.5 kDa
• Strain: C57BL/6
• Reactivity: Mouse
• Host: Mouse
• Application: ELISA ; FACS ; IF ; Fn ; WB
• Description: Monoclonal antibody directed against 5T4, with use as a prognostic aid of tumours. Background and Research Application The murine 5T4 monoclonal antibody, clone B3F1, identifies the human 5T4 oncofoetal antigen, also known as trophoblast glycoprotein. 5T4 is a highly glycosylated protein expressed by trophoblast and a few specialized adult epithelia and is an antagonist of the Wnt/ ?-catenin signalling pathway. Up-regulation of 5T4 expression in some cancers is associated with poor clinical outcome; overexpression of human 5T4 cDNA in epithelial cells can alter their morphology and motility, supporting a role for such functions in cancer and development. 5T4 has limited expression in normal tissues, but is widespread in tumours, thus its expression is used as a prognostic aid. 5T4 is typically found in colorectal, ovarian and gastric tumours. 5T4 is a potential target for T cells, in antibody-directed immunotherapy.
• Immunogen: Q9Z0L0
• Immunogen uniprot id: Q9Z0L0
• Isotype: IgG2a

Target Details

• Target: 5T4 oncofetal glycoprotein
• Molecular weight: 46.5 kDa
• Target background: Monoclonal antibody directed against 5T4, with use as a prognostic aid of tumours. Background and Research Application The murine 5T4 monoclonal antibody, clone B3F1, identifies the human 5T4 oncofoetal antigen, also known as trophoblast glycoprotein. 5T4 is a highly glycosylated protein expressed by trophoblast and a few specialized adult epithelia and is an antagonist of the Wnt/ ?-catenin signalling pathway. Up-regulation of 5T4 expression in some cancers is associated with poor clinical outcome; overexpression of human 5T4 cDNA in epithelial cells can alter their morphology and motility, supporting a role for such functions in cancer and development. 5T4 has limited expression in normal tissues, but is widespread in tumours, thus its expression is used as a prognostic aid. 5T4 is typically found in colorectal, ovarian and gastric tumours. 5T4 is a potential target for T cells, in antibody-directed immunotherapy.

Applications

• Application: ELISA ; FACS ; IF ; Fn ; WB

Handling

• Format: Liquid
• Concentration: 1 mg/ml
• Unit size: 100 ug
• Storage buffer: PBS with 0.02% azide
• Storage conditions: Store at -20° C frozen. Avoid repeated freeze / thaw cycles
• Shipping conditions: Shipping at 4° C

Related Tools

• Related tools: Anti-5T4 [B1C3] ; Anti-5T4 [B5C9] ; Anti-5T4 [B5C12]

References

• Castro et al. 2012. Cancer Immunol Immunother. 61(7):1005-18. PMID: 22127365.
• Southgate et al. 2010. PLoS One. 5(4):e9982. PMID: 20376365.
• CXCR4 mediated chemotaxis is regulated by 5T4 oncofetal glycoprotein in mouse embryonic cells.
• Ward et al. 2003. J Cell Sci. 116(Pt 22):4533-42. PMID: 14576347.
• The 5T4 oncofoetal antigen is an early differentiation marker of mouse ES cells and its absence is a useful means to assess pluripotency.
• Woods et al. 2002. Biochem J. 366(Pt 1):353-65. PMID: 12003637.
• Characterization of the murine 5T4 oncofoetal antigen: a target for immunotherapy in cancer.