Anti-5T4 [B1C3]

The murine 5T4 monoclonal antibody, clone B1C3, defines the human 5T4 oncofoetal antigen, a highly glycosylated protein expressed by trophoblast and a few specialized adult epithelia.

Contributors

Inventor Institute
Peter Stern Cancer Research UK, Manchester Institute
SKU: 152575
Product description: The murine 5T4 monoclonal antibody, clone B1C3, defines the human 5T4 oncofoetal antigen, a highly glycosylated protein expressed by trophoblast and a few specialized adult epithelia. Up-regulation of 5T4 expression in some cancers is associated with poor clinical outcome; overexpression of human 5T4 cDNA in epithelial cells can alter their morphology and motility, supporting a role for such functions in cancer and development.
Alternate name: Trophoblast Glycoprotein; 5T4 Oncofetal Trophoblast Glycoprotein; Wnt-Activated Inhibitory Factor 1; 5T4 Oncotrophoblast Glycoprotein; 5T4 Oncofetal Antigen; WAIF1; M6P1; 5T4; 5T4AG
Conjugation: Unconjugated
Isotype: IgG2a
Molecular weight: 46.5 kDa
Cat. #: 152575
Provenance and Ownership: The Anti-5T4 [B1C3] is owned by Cancer Research UK. CancerTools supplies the Anti-5T4 [B1C3] under licence from Cancer Research UK and is the authorised source for its distribution. Sourcing Anti-5T4 [B1C3] directly from the IP owner ensures authenticity, provenance and compliance with all applicable licensing and research use requirements.
Tool sub type: Primary antibody
Unit size: 100 ug
Research Fields: Cancer; Stem cell biology
Application: ELISA ; FACS ; Fn
Target: 5T4 oncofetal glycoprotein
Reactivity: Mouse
Clone: B1C3
Host: Mouse
Class: Monoclonal
Target background: The murine 5T4 monoclonal antibody, clone B1C3, defines the human 5T4 oncofoetal antigen, a highly glycosylated protein expressed by trophoblast and a few specialized adult epithelia. Up-regulation of 5T4 expression in some cancers is associated with poor clinical outcome; overexpression of human 5T4 cDNA in epithelial cells can alter their morphology and motility, supporting a role for such functions in cancer and development.
Format: Liquid
Concentration: 1 mg/ml
Storage buffer: PBS with 0.02% azide
Storage conditions: -15° C to -25° C
Shipping conditions: Dry ice
References:

Southgate et al. 2010. PLoS One. 5(4):e9982. PMID: 20376365.

CXCR4 mediated chemotaxis is regulated by 5T4 oncofetal glycoprotein in mouse embryonic cells.

Ward et al. 2003. J Cell Sci. 116(Pt 22):4533-42. PMID: 14576347.

The 5T4 oncofoetal antigen is an early differentiation marker of mouse ES cells and its absence is a useful means to assess pluripotency.

Woods et al. 2002. Biochem J. 366(Pt 1):353-65. PMID: 12003637.

Characterization of the murine 5T4 oncofoetal antigen: a target for immunotherapy in cancer.

Images

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Summary of the IgG subclasses of five m5T4 specific monoclonal antibodies (mAb; made in 5T4KO mice) recognizing distinct epitopes in the proximal and distal 5T4 extracellular LRR containing domains (Southgate et al. 2010. PLoS One. 5(4)

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